丙型肝炎基因型分布特点及流行分析  

Genotype distribution and epidemiological investigation of HCV

作　　者：刘剑荣 黄永建[1] 夏洪娇[1] 张勇[1] 

机构地区：[1]江西省萍乡市人民医院检验科,337055

出　　处：《国际检验医学杂志》2010年第12期1347-1348,共2页International Journal of Laboratory Medicine

摘　　要：目的分析萍乡地区丙型肝炎患者的丙型肝炎病毒(HCV)基因型,了解本地区丙型肝炎分布及流行情况,为慢性丙型肝炎的诊断、治疗及预防提供有力的依据。方法利用门诊及住院患者血清HCV抗体阳性标本,经荧光定量PCR检测HCVRNA阳性标本102例,用基因芯片进行基因分型检测。结果检测HCV感染标本102例,共检出7种基因亚型,分别为1b、6型、2a、1b+2a、1b+3a、3a和3b,其中1b占72.6,6型占8.8,2a占7.8,1b+2a占5.9,1b+3a占2.9,3a占1.0,3b占1.0。结论萍乡地区HCV基因型主要为1b,6型为第2常见亚型,混合基因型感染较多;有吸毒史、外伤史、输血史三者合计76例,占74.5。安源区和湘东区所占比例较大。Objective To analyze the HCV genotype in the patients with chronic C hepatitis in Pingxiang and understand the distribution and prevalence of C hepatitis C in this region. To provide potent basis for the diagnosis,treatment and prevention of C hepatitis. Methods 102 cases of serum specimens, containing anti--HCV antibody, from inpatients and outpatients were detected for HCV RNA by real time PCR and for genotype by gene chips. Results In the 102 specimens with HCV infection,there were 7 gene subtypes detected,including 1b. type 6, 2a. 1b ＋2a, 1b＋3a, 3a and 3b,with proportion of 72.6% ,8.8% ,7.8% ,5.9%,2. 9%,1.0% and 1.0%, respectively. Conclusion The mainly genotype of HCV in Pingxiang is l b, and type 6 is the second most common type. Infection of mixed genotype is common. 76 （74.5 %） cases of patients enrolled in this study had history of drug abuse,trauma or blood transfusion. The larger proportion of patients were from Xiangdong and Anyuan district.

关 键 词：肝炎病毒 寡核苷酸序列分析 基因型 流行病学 

分 类 号：R446.61[医药卫生—诊断学] R512.63[医药卫生—临床医学]