Synthesis of a Kind of Temperature-responsive Cell Culture Surface for Corneal Sheet  

作　　者：Yanqing Guan Zhibin Li Xin Wang Xiaoli Ni Aini Yang Junming Liu 

机构地区：[1]School of Life Science, South China Normal University, Guangzhou 510631, China [2]School of Physics, South China Normal University, Guangzhou 510631, China [3]Laboratory of Solid State Microstructures, Nanjing University, Nanjing 210093, China [4]International Center for Materials Physics, Chinese Academy of Sciences, Shenyang 110016, China

出　　处：《Journal of Materials Science & Technology》2010年第12期1119-1126,共8页材料科学技术（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos.30970731 and 50832002);the Natural Science Foundation of Guangdong Province(No.9151063101000015)

摘　　要：In this study, acrylic acid （AA） and 4-azidoaniline were used to modify poly （N-isopropylacrylamide） （NIPAAm） in order to fabricate temperature-responsive surface for corneal epithelia cell adhesion and detachment. First, NIPAAm was copolymerized with acrylic acid. Then, the copolymer was coupled with azidoaniline to synthesize AzPhPIA, derivative of p（NIPAAm-co-AA）, which possesses both thermo- and photo-sensitivities. Second, the synthesized copolymer was characterized by high performance liquid chromatography （HPLC）, Fourier transform infrared （FTIR） and a CHN analyzer. The thermo-sensitivity was characterized by temperature reducing experiment, contact angle measurement and low critical solution temperature （LCST） testing. Third, the derivatized copolymer was immobilized by photolithography on a polystyrene plate, and then the surface characterization of AzPhPIA-coated polystyrene plate （PSt） was measured by electron spectroscopy for chemical analysis （ESCA）. The thermo-sensitivity and cytocompatibility of the AzPhPIA-coated PSt were investigated by corneal epithelial cells culture. The results revealed that the AzPhPIA-coated PSt exhibited good cytocompatibility and cell detachability when temperature decreased.In this study, acrylic acid （AA） and 4-azidoaniline were used to modify poly （N-isopropylacrylamide） （NIPAAm） in order to fabricate temperature-responsive surface for corneal epithelia cell adhesion and detachment. First, NIPAAm was copolymerized with acrylic acid. Then, the copolymer was coupled with azidoaniline to synthesize AzPhPIA, derivative of p（NIPAAm-co-AA）, which possesses both thermo- and photo-sensitivities. Second, the synthesized copolymer was characterized by high performance liquid chromatography （HPLC）, Fourier transform infrared （FTIR） and a CHN analyzer. The thermo-sensitivity was characterized by temperature reducing experiment, contact angle measurement and low critical solution temperature （LCST） testing. Third, the derivatized copolymer was immobilized by photolithography on a polystyrene plate, and then the surface characterization of AzPhPIA-coated polystyrene plate （PSt） was measured by electron spectroscopy for chemical analysis （ESCA）. The thermo-sensitivity and cytocompatibility of the AzPhPIA-coated PSt were investigated by corneal epithelial cells culture. The results revealed that the AzPhPIA-coated PSt exhibited good cytocompatibility and cell detachability when temperature decreased.

关 键 词：N-ISOPROPYLACRYLAMIDE Low critical solution temperature Tissue engineering Corneal epithelial cells 

分 类 号：O648.17[理学—物理化学] Q813.11[理学—化学]