新疆伊犁哈萨克地区无偿献血人群中HIV HBV和HCV的核酸检测  

Nucleic acid testing (NAT) for HIV,HBV and HCV in the blood donors in Xinjiang·Yili·Kazak Autonomous Region

作　　者：赵全壁[1] 蔡淑霞 刘玉磊[1] 周克隆 杜文阁 杨友华 徐维四[1] 袁青 邵一鸣 马丽英[1] 

机构地区：[1]中国疾病预防控制中心性病艾滋病预防控制中心国家传染病重点实验室,北京102206 [2]新疆伊犁哈萨克自治州中心血站,新疆835000 [3]上海科华生物工程股份有限公司,上海200233

出　　处：《中国艾滋病性病》2010年第6期539-541,606,共4页Chinese Journal of Aids & STD

基　　金："十一五"国家科技重大专项项目(课题编号:2008ZX10001-013;2009DFB30420)

摘　　要：目的了解新疆伊犁哈萨克地区健康献血人群中,艾滋病病毒(HIV)、乙型肝炎病毒(HBV)和丙型肝炎病毒(HCV)"窗口期"病人的发生率,对无偿献血人群的血标本进行HIV、HBV和HCV核酸检测(NAT)。方法从无偿献血人群血浆中同时提取HIV、HBV和HCV核酸,采用荧光实时聚合酶链反应(PCR)进行检测,同步利用酶联免疫吸附试验(ELISA)检测HIV、HCV和HBV抗原或抗体。结果 5 751份献血员血标本中,检出HBV"窗口期"标本3份,检出率为0.087%;检出HCV"窗口期"标本1例,检出率为0.017%,未检测出HIV"窗口期"标本。结论核酸检测可以有效检测出无偿献血人群中HIV、HBV和HCV的"窗口期"标本。新疆伊犁哈萨克地区无偿献血人群中存在HBV、HCV的"窗口期"潜在感染危险,应考虑在该地区无偿献血人群中进行核酸检测。Objective To study nucleic acid testing（NAT）for HIV/HBV /HCV among blood donors in Xinjiang Yili Kazak Autonomous Region Methods The nucleic acid from blood donor plasma was extracted.HIV,HBV and HCV were detected using fluorescent real-time PCR.Meanwhile,HBVsAg,anti-HIV and anti-HCV antibodies were detected using ELISA method respectively.Results Among 5751 samples,there were 3,1 and 0 ＂window period＂ samples for HBV,HCV and HIV respectively,with the ＂window period＂ incidence of 0.087% for HBV,0.017% for HCV and 0% for HIV in these populations.Conclusions Nucleic acid testing（NAT）can effectively detect the ＂window period＂ samples of blood donors.This study suggests that there are still a risk from current donor screening process with immune-testing in XinJiang · Yili· Kazak Autonomous Region,and NAT should be taken into account as a complementary assay method for donor screening.

关 键 词：无偿献血人群 核酸检测 艾滋病病毒 乙型肝炎病毒 丙型肝炎病毒 

分 类 号：R512.91[医药卫生—内科学]