姜黄素对人胃癌SGC-7901细胞增殖及COX-2mRNA和COX-2蛋白表达的影响  

Effects of Curcumine on the Proliferation of Gastric Cancer Cell Line SGC-7901 and Expressions of COX-2mRNA and COX-2 Protein

作　　者：司宏[1] 徐艳艳[1] 

机构地区：[1]江苏省南通市肿瘤医院,南通226361

出　　处：《中国医院用药评价与分析》2010年第12期1089-1091,共3页Evaluation and Analysis of Drug-use in Hospitals of China

摘　　要：目的:探讨姜黄素对人胃癌SGC-7901细胞增殖及对环氧酶-2(C0X-2)基因及蛋白表达的影响。方法:应用MTT法考察浓度姜黄素对胃癌SGC-7901细胞增殖的影响;应用RT-PCR、Western-blotting法检测COX-2 mRNA及蛋白的表达。结果:5～80μg.mL-1姜黄素对胃癌SGC-7901细胞增殖的抑制作用呈剂量依赖性和时间依赖性。姜黄素对COX-2mRNA和COX-2蛋白的表达有不同程度的抑制作用,随着剂量的增加,抑制作用增强,较高浓度(20～80μg.mL-1)的姜黄素对COX-2 mRNA和COX-2蛋白的表达有显著抑制作用(P<0.01)。结论:姜黄素可能通过抑制COX-2 mRNA和COX-2蛋白的表达抑制胃腺癌细胞SGC-7901的增殖,可能成为一种胃癌预防剂。OBJECTIVE：To investigate the effects of curcumin on the proliferation of gastric cancer cell line SGC-7901 and the gene /protein expressions of cyclooxygenase-2（COX-2）.METHODS：The effect of different concentration of curcumin on the proliferation of gastric cancer cell line SGC-7901 was determined by MTT method.The expressionsof COX-2 mRNA and COX-2 protein were detected by reverse transcriptase polymerase chain reaction（RT-PCR）andWestern blot assay,respectively.RESULTS：The inhibitory effect of curcumin（5-80μg·mL-1）on the proliferation ofgastric cancer cell line SGC-7901 was in a dose-and time-dependent manner.Curcumin showed different degree ofinhibitory effects on the expressions of COX-2 mRNA and COX-2 protein in a dose-dependent manner;at a highconcentration（20-80μg·mL-1）,curcumin showed significant inhibitory action on the expressions of COX-2 mRNA and COX-2 protein（P0.01）.CONCLUSION：Curcumin can inhibit the proliferation of SGC-7901 through down-regulation on the expressions of COX-2 mRNA and COX-2 protein,which is expected to become a preventative agentfor gastric cancer.

关 键 词：姜黄素 环氧酶-2 增殖 

分 类 号：R979.1[医药卫生—药品]