坐骨神经缺损1周背根神经节组织中microRNA的表达变化  

Altered microRNA expression following sciatic nerve resection for 1 week

作　　者：于彬[1] 周松林[1] 钱天梅[1] 王勇军[1] 丁斐[1] 顾晓松[1] 

机构地区：[1]南通大学神经再生重点实验室,南通226001

出　　处：《南通大学学报（医学版）》2010年第6期410-412,共3页Journal of Nantong University(Medical sciences)

基　　金：863重大项目(2006AA02A128);国家自然科学基金(30870811);江苏省自然科学创新学者攀登项目(BK2008010);江苏省自然科学基金(BK2010283)

摘　　要：目的:研究大鼠坐骨神经缺损后背根神经节中microRNA(miRNA)的表达变化。方法:采用miRNA芯片检测神经缺损0 d(对照组)和7 d(实验组)背根神经节组织中miRNA的表达情况,应用Real time Taqman PCR对芯片结果进行验证。结果:实验组与对照组比较发生上调1.4倍和下调>1%以上的miRNA分别有13个和5个,Real time TaqmanPCR检测的4个miRNA的表达变化与芯片一致(上调:miR-21,miR-221;下调:miR-500,miR-551b)。结论:大鼠坐骨神经缺损1周后背根神经节组织中miRNA的表达谱发生改变。Objective： To profile the expression of microRNAs（miRNAs） in the dorsal root ganglia（DRG） after resection of the sciatic nerve for 1 week in rats.Methods： We examined the expression of miRNAs in a sciatic nerve resection model at 0 day and 7 days using an Agilent rat miRNA array.The miRNA expression was confirmed by Real time Taqman PCR.Results： 13 and 5 miRNAs were significantly up-regulated by 1.4-fold and down-regulated by 1% in the DRG after 1 week of resection,respectively.The expression of the selected miRNAs analyzed by Real time Taqman PCR was in agreement with the microarray data（up-regulated： miR-21,miR-221;down-regulated： miR-500,miR-551b）.Conclusion： This study demonstrated a different involvement of miRNAs in the DRG after resection of the sciatic nerve in a rat model.

关 键 词：MICRORNA 神经缺损 背根神经节 

分 类 号：R651.3[医药卫生—外科学]