狼疮性肾炎患者检测血、尿中性粒细胞明胶酶相关脂质运载蛋白的临床研究  

Clinical research on plasma and urine neutrophil gelatinase-associated lipocalin of lupus nephritis

作　　者：谢伟基[1] 张夏兰[1] 张丽玲[1] 洪楷[1] 肖再雄[1] 陈东晓[1] 耿义群[1] 

机构地区：[1]汕头大学医学院第二附属医院肾内科,515041

出　　处：《中国医师进修杂志》2010年第36期1-3,共3页Chinese Journal of Postgraduates of Medicine

基　　金：广东省科技计划（20078031503006）

摘　　要：目的 测定狼疮性肾炎（LN）患者和健康成年人的血、尿中性粒细胞明胶酶相关脂质运载蛋白（NGAL）并分析其临床意义.方法 选取符合1997年美国风湿病学会修订的系统性红斑狼疮的诊断标准,同时合并有不同程度肾损害而确诊合并LN患者35例作为研究组,选取年龄、性别相匹配的健康成年人30例作为对照组,采用酶联免疫吸附技术（ELISA）分别测定血、尿NGAL的水平.另依据SLEDAI-2K的肾病活动指数将研究组患者分为肾病活动组（25例）与非肾病活动组（10例）,并比较血、尿NGAL水平.结果 研究组患者尿NGAL水平为（78.94±81.97）μg/L,明显高于对照组的（28.50±18.08）μg/L,差异有统计学意义（P=0.002）.肾病活动组尿NGAL水平为（92.90±94.88）μg/L,明显高于非肾病活动组的（48.20±24.77）μg/L,差异有统计学意义（P=0.049）,两组血NGAL水平比较差异无统计学意义（P＞0.05）.结论 LN患者尿NGAL可作为肾病活动性的指标,其升高原因可能与肾小管病变有关.Objective To study the clinical significance ofneutrophil gelatinase-associated lipocalin （NGAL）, which in serial plasma and urine samples was measured in participants with lupus nephritis （LN）and healthy persons. Methods NGAL in serial plasma and urine samples was measured by enzyme-linked immunosorbent assay （EL.ISA） in 35 patients with LN by 1997 ACR systemic lupus erythematosus （SLE）standard with varied degree of kidney damage and 30 healthy persons with matching sex and age in physical examination center. Disease activity was measured by the SLE disease activity index （SLEDAI-2K）,and 35LN patients were classified in active group and （25 cases） non-active group （10 cases） according to the SLEDAI-2K. Results Urinary NGAL were significantly increased in LN patients [（78.94 ± 81.97） μg/L]compared with healthy persons[（28.50 ± 18.08） μ g/L] （P = 0.002）. And urinary NGAL were significantly increased in active group [（92.90 ± 94.88） μg/L] compared with non-active group [（48.20 ± 24.77）μ g/L] （P = 0.049）. NGAL in serial plasma had no statistically significant difference between active group and non-active group （P 〉0.05）. Conclusions NGAL in urine but not in plasma represents a novel biomarker for renal disease activity in LN. The increase might be related to renal tubule pathological changes.

关 键 词：狼疮肾炎 血液 尿△ 中性粒细胞明胶酶相关脂质运载蛋白 

分 类 号：R692[医药卫生—泌尿科学]