人源性抗日本血吸虫肌球蛋白Fab噬菌体抗体体外杀童虫作用  

Killing effect of humanized Fab fragment phage antibody against myosin of Schistosoma japonica to schistosomula in vitro

作　　者：任翠平[1] 程晓虎[2] 童晶晶[2] 汪洁[2] 成晓[2] 廖法学[2] 雷黎[3] 刘森[1] 沈际佳[1] 

机构地区：[1]安徽病原生物学省级实验室,安徽医科大学病原生物学教研室,合肥230032 [2]安徽医科大学第一临床学院,合肥230032 [3]安徽中医学院第一附属医院,合肥230031

出　　处：《中国人兽共患病学报》2010年第12期1101-1104,共4页Chinese Journal of Zoonoses

基　　金：安徽省自然科学基金(050430805);安徽医科大学科研基金(2010xkj022)联合资助

摘　　要：目的观察人源性抗日本血吸虫肌球蛋白Fab噬菌体抗体体外杀伤血吸虫童虫作用。方法利用日本血吸虫肌球蛋白(Sj myosin)对人源性抗日本血吸虫Fab噬菌体抗体库进行富集筛选,获得抗Sjmyosin的特异性噬菌体抗体,将该抗体和巨噬细胞或补体与日本血吸虫童虫共培养,测定体外杀伤日本血吸虫童虫作用。结果将抗Sj myosin噬菌体抗体和巨噬细胞及补体与血吸虫童虫共培养时,在培养后24h即出现杀伤作用(童虫死亡率为4.69%),48h童虫死亡率达84.06%,96h童虫死亡率达89.38%。结论人源性抗Sj myosin Fab噬菌体抗体具有体外杀伤日本血吸虫童虫活性。To observe killing effect of humanized Fab fragment phage antibody against Myosin of Schistosoma japonica to schistosomula in vitro.We used myosin to enrich humanized Fab fragment phage antibody library,and acquired humanized Fab fragment phage antibody against myosin;then measured its killing effect after the antibody cultured with schistosomula.The result showed that the antibody began had killed schistosomula 24h after incubation with schistosomula.It indicated that the humanized Fab fragment phage antibody has killing effect on Schistosoma.

关 键 词：日本血吸虫 噬菌体抗体 血吸虫童虫 体外杀伤作用 

分 类 号：R383.2[医药卫生—医学寄生虫学]