缺氧诱导因子1α和微血管密度在黏液表皮样癌中的表达和意义  

Study on the expression of hypoxia inducible factor-la and microvessel density in mucoepidcrmoid carcinoma

作　　者：欧阳可雄[1] 梁军[2] 黄志权[3] 林宇静[4] 韦丽芬[1] 

机构地区：[1]广州医学院口腔医院口腔颌面外科,510140 [2]中山大学附属第五医院口腔颌面外科 [3]中山大学孙逸仙纪念医院口腔颌面外科 [4]中山大学附属第五医院病理科

出　　处：《中华口腔医学研究杂志（电子版）》2010年第6期23-26,共4页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：广东省医学科研基金（B2010146）;珠海市科技计划项目（20108040102014）

摘　　要：目的研究缺氧诱导因子1α（HIF-1α）和微血管密度（MVD）在黏液表皮样癌（MEC）中的表达,探讨两者与MED临床病理类型的关系。方法应用免疫组化SP法检测30例正常唾液腺组织、38例高分化MEC及26例低分化MEC中HIF-1α和MVD的表达。结果 HIF-1α的阳性表达率和MVD计数在正常唾液腺组织、高分化MEC及低分化MEC中的表达均依次增强,组间差异均有统计学意义（P〈0.05）;MEC中MVD计数随着HIF-1α表达的增强而升高（P〈0.05）;发生淋巴结转移组的MEC中HIF-1α的阳性表达率和MVD计数均高于非转移组（P〈0.05）。结论 HIF-1α的表达和MVD与MEC的分化程度和淋巴转移程正相关。Objective To investigate the expression of hypoxia inducible factor-1α（HIF-1α） and microvessel density （MVD） in mucoepidermoid carcinoma （MEC） and explore their relationship with the clinicopathological characters of MEC. Methods Streptavidin-peroxidase （SP） immunochemical method was applied to detect the expression of HIF-1α; MVD was counted by anti-CD34 antibody in 30 cases of normal salivary tissues, 38 cases of well-differentiated MEC tissues, and 26 cases of poorly-differentiated MEC tissues. Results Positive rate of HIF-1α expression and MVD count progressively increased from the normal salivary gland tissues to the well-differentiated MEC tissues and the poorly-differentiated MEC tissues（P 〈 0.05）. MVD count tended to depend on the HIF-1α expression levels in MEC（P 〈 0.05）; the value of lymph-metastasis group is higher than that of non-lymph-metastasis group （P 〈 0.05）. Conclusions The expression of HIF-1α and MVD are positively related with the cell differentiation and lymphatic metastasis of mucoepidermoid carcinoma.

关 键 词：缺氧诱导因子-1Α 微血管密度 黏液表皮样癌 

分 类 号：R543.2[医药卫生—心血管疾病]