曲美他嗪对2型糖尿病模型大鼠心室肌Ito通道表达的影响研究  

Effect of Trimetazidine on the Expression of Ito Channel in the Left Ventricles of Type 2 Diabetic Rats

作　　者：何莉[1] 向玉鸾[1] 佘强[1] 

机构地区：[1]重庆医科大学附属第二医院心血管内科,重庆市400010

出　　处：《中国药房》2011年第5期389-392,共4页China Pharmacy

基　　金：国家自然科学基金资助项目(30971213);重庆市卫生局重点项目(20090113)

摘　　要：目的:探讨曲美他嗪(TMZ)对2型糖尿病模型大鼠心室肌Ito通道α亚单位(Kv1.4、Kv4.2、Kv4.3)表达的影响。方法:大鼠高脂喂养1月后,一次性腹腔注射链脲佐菌素35mg·kg-1建立2型糖尿病模型,建模成功后,随机分为治疗组(高脂饮食,灌胃TMZ10mg·kg-1)和模型组(高脂饮食,灌胃生理盐水),每组10只,给予相应药物,每天1次,4周后分别用实时荧光定量聚合酶链式反应技术(Real-timePCR)、蛋白免疫印迹法(Westernblot)检测左心室Kv1.4、Kv4.2、Kv4.3mRNA和蛋白质的表达水平,另设正常对照组进行比较。结果:与正常对照组比较,模型组大鼠左心室Kv1.4mRNA和蛋白质表达明显升高,Kv4.2、Kv4.3mRNA和蛋白质表达明显降低(P均<0.01);与模型组比较,治疗组大鼠左心室Kv1.4mRNA和蛋白质表达明显降低,Kv4.2、Kv4.3mRNA和蛋白质表达明显升高(P均<0.05)。结论:TMZ可逆转2型糖尿病模型大鼠心室肌Ito通道α亚单位表达的变化。OBJECTIVE： To investigate the effects of trimetazidine （TMZ） on the expressions of α subunit of Ito channel （Kv1.4, Kv4.2, Kv4.3） in the left ventricle of type 2 diabetic rats. METHODS： The type 2 diabetic model was established by feeding high fat diet for a month and single injection with streptozotocin 35 mg·kg-1 peritoneally. Model rats were randomly divided into treatment group （high fat diet＋intragastric administration of TMZ 10 mg·kg-1） and model group （high fat diet＋intragastric administration of normal saline） with each group of 10 rats. Both groups were given relevant drugs once a day for 4 weeks. Kv1.4, Kv4.2, Kv4.3 mRNA and protein levels were detected using real-time polymerase chain reaction （real-time PCR） and Western blot 4 weeks later. RESULTS： Compared with normal control group, the expression levels of Kv1.4 mRNA and protein in left ventricle of model group was increased significantly, while the expression levels of Kv4.2, Kv4.3 mRNA and protein were decreased significantly （P0.01）. Compared with model group, the expression levels of Kv1.4 mRNA and protein in left ventricle of treatment group were lowered significantly, while the expression levels of Kv4.2, Kv4.3 mRNA and protein were heightened significantly （P0.05）. CONCLUSION： TMZ may reverse the variance of α subunit of Ito channel in the left ventricle of type 2 diabetic rats.

关 键 词：曲美他嗪 2型糖尿病 大鼠 心室肌Ito通道 

分 类 号：R587.1[医药卫生—内分泌] R541.7[医药卫生—内科学]