白藜芦醇甙对新生大鼠HIBD后脑组织细胞间黏附分子-1表达的影响  

Effects of polydatin on intercellular cell adhesion molecule-1 in cortex of neonatal rats with hypoxicischemia brain damage

作　　者：曲云霞[1] 李开花[2] 何绘敏[3] 王朝晖[4] 徐立新[1] 

机构地区：[1]大连医科大学附属第二医院儿科,116023 [2]郴州市第一人民医院新生儿科,423000 [3]大连医科大学附属第二医院癫痫中心,116023 [4]大连医科大学附属第二医院实验中心,116023

出　　处：《国际儿科学杂志》2011年第1期94-95,F0003,F0004,共4页International Journal of Pediatrics

基　　金：辽宁省自然科学基金资助项目（20072172）

摘　　要：目的 探讨白藜芦醇甙（PD）对缺氧缺血性脑损伤（HIBD）新生大鼠脑组织细胞间黏附分子（ICAM-1）表达的影响.方法 7日龄SD大鼠54只随机分为假手术组、HIBD自然恢复组和PD治疗组,采用经典的Rice方法制备HIBD动物模型,用免疫组织化学方法观察各组大鼠脑皮质微血管内皮ICAM-1的表达.结果 假手术组少许脑实质中微血管显示阳性免疫染色.HIBD组缺氧缺血后6h ICAM-1 免疫染色阳性血管数明显增多,12h达高峰,24h ICAM-1表达仍然持续在较高水平;PD治疗组与HIBD组比较,缺氧缺血后6h、12h、24h脑微血管内皮ICAM-1表达明显减少（P〈0.05或P〈0.01）.结论 在HIBD早期使用PD能降低脑微血管内皮ICAM-1的表达,抑制脑内炎症反应,对缺氧缺血后大脑具有神经保护作用.Objective To investigate the protective effects and possible mechanisms of polydatin（PD） on hypoxic-ischemia brain damage（HIBD） in neonatal rat by means of the expression of intercellular cell adhesion molecule（ ICAM）-1 in cortex. Methods Fifty-four SD rats were divided into 3 groups at random, shame group （no HIBD）, HIBD group （no medication） ,and PD treatment group. 7day-old rat＇s HIBD model was established by Rice＇s method. ICAM-1 expression in brain after HIBD was measured in different time by Immunohistochemitry technique. Results In sham group, there were less brain microvessel immunostained positively. In HIBD group,the number of ICAM-1 immuno-positive staining blood vessels increased significantly after 6h, 12h reached peak point. ICAM-1 immunoreactive staining of blood vessels levels continued in the peak after 24h. In PD treatment group, ICAM-1 expression on brain microvascular endothelial decreased after HIBD 6h, 12h, 24h, which was significant compared with HIBD group（ P 〈 0. 05 or P 〈 0. 01 separately）. Conclusion The expression of ICAM-1 was involved in the procedure induced by hypoxic-ischemia. After HIBD, polydatin would downregulate ICAM-1 expression in cerebral microvascular endothelial, and inhibite the inflammatory response.

关 键 词：白藜芦醇甙 缺氧缺血性脑损伤 细胞间黏附分子 

分 类 号：R285.5[医药卫生—中药学]