荧光分析法测定兔血浆中蛇葡萄素浓度(英文)  

Development of a Fluorescence Analysis Method for Determining Ampelopsin in Rabbit Plasma

作　　者：黄仁杰[1] 邓元荣 

机构地区：[1]福建卫生职业技术学院药学系,福州350101

出　　处：《中国现代应用药学》2011年第1期70-74,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：福建省卫生厅青年科研基金资助项目(2006-1-26)

摘　　要：目的建立一种用于测定兔血浆中蛇葡萄素浓度的荧光分析方法。方法经单剂量静脉给药后,收集家兔血样并经乙酸乙酯萃取,加入三氯化铝溶液后在激发波长442 nm和发射波长502 nm下进行血药浓度检测。结果血浆中蛇葡萄素浓度在0.5~36.6μg.mL-1内与荧光强度呈良好线性关系,r=0.999 6。检测限为70 ng.mL-1,日内、日间精密度在98.78%~103.57%之间,RSD均小于8.25%。结论该方法简便、快速、准确,可用于蛇葡萄素的药代动力学研究。OBJECTIVE To establish a fluorescence analysis method for determining ampelopsin in rabbit plasma.METHODS After intravenous administration with ampelopsin at a single dose,the blood samples were collected and extracted with ethyl acetate.Ampelopsin concentrations were determined with fluorescence detection exciting at 442 nm and emission at 502 nm.RESULTS The calibration curve showed a linear response over the concentration range of 0.5-36.6 μg·mL-1（r=0.999 6）.The limit of detection was 70 ng·mL-1.The recovery was between 98.78% and 103.57%.The relative standard deviations of intra-day and inter-day were less than 8.25%.CONCLUSION A rapid,simple and accurate method has been successfully applied to a pharmacokinetics study of ampelopsin in rabbits.

关 键 词：蛇葡萄素 荧光分析法 生物分析 药代动力学 

分 类 号：R969.11[医药卫生—药理学]