肺泡表面活性物质蛋白B基因多态性与支气管肺发育不良易感性的关系  

Relationship between Polymorphism of Surfactant Protein B Gene and Bronchopulmonary Dysplasia

作　　者：曾凌空[1,2] 李文斌[1] 潘睿[1] 单瑞艳[1] 刘汉楚[2] 周玉荣[1] 张佳[1] 常立文[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院儿科,武汉430030 [2]武汉市儿童医院新生儿科,武汉430016

出　　处：《实用儿科临床杂志》2011年第2期132-134,共3页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金(30872795)

摘　　要：目的了解肺泡表面活性物质蛋白B(SPB)-18A/C和SPB1580C/T基因多态性与支气管肺发育不良(BPD)易感性的关系。方法应用聚合酶链反应-限制性片段长度多态分析技术及基因测序技术检测57例BPD患儿及103例同胎龄无BPD早产儿的SPB-18及SPB1580基因多态性,比较2组SPB-18及SPB1580基因型和基因分布的差异。结果 1.SPB-18A/C基因型在BPD组与对照组AA、AC、CC基因型频率分别为14.0%、45.6%、40.4%和4.9%、31.1%、64.1%;A等位基因增加新生儿患BPD的风险:AC/CC与AA/CC的OR值分别为2.3(95%CI1.2～4.7,P=0.02)和4.7(95%CI1.4～16.2,P=0.01);2.SPB1580C/T基因型在对照组和BPD组TT、CT、TT分布分别为6.8%、45.6%、47.6%和12.3%、40.4%、47.4%,对照组和BPD组比较差异无统计学意义(χ2=1.50,P>0.05)。结论中国武汉汉族人中SPB-18基因多态性是BPD的危险因素,基因型为AA者是BPD易感人群。SPB1580C/T基因多态性与BPD发病无明显相关性。Objective To study the relationship between polymorphism of surfactant protein B（SPB）and susceptibility of bronchopulmonary dysplasia（BPD）.Methods The genetic polymorphism of SPB gene-18A/C and SPB 1580C/T,coded for SPB,were analyzed and then compared by using polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）and gene sequencing in 57 children with BPD,and 103 children born at the same gestational without BPD.Results 1.The SPB-18 AA,AC,and CC genotype frequencies in BPD patients and control group were 14.0%,45.6% and 40.4% vs 4.9%,31.1% and 64.1%,respectively;A allele increased the risk of getting neonatal BPD as the following：the OR of AC/CC and AA/CC was 2.3（95% CI 1.2-4.7,P=0.02）and 4.7（95% CI 1.4-16.2,P=0.01）;2.The SPB 1580 TT,CT and TT genotype frequencies in BPD patients were 6.8%,45.6%,47.6%,respectively and 12.3%,40.4%,and 47.4%,respectively in control group,there was no significant difference between both groups（χ2=1.50,P0.05）.Conclusions SPB-18A/C gene polymorphism in Wuhan pediatric population is a susceptibility factor for BPD and AA is the genotype that increases the risk for BPD.However,no relationship is found between SPB 1580 locus and BPD.

关 键 词：肺泡表面活性物质蛋白 基因多态性 支气管肺发育不良 易感性 

分 类 号：R722.6[医药卫生—儿科]