磷脂抗体阳性红斑狼疮患者周围血T细胞受体基因表达初探  

Aprimary study on TCR Vβusage of peripheral blood lymphocyte patients with positive antiphospholipid antibody ofsystemiclupus erythematosus

作　　者：王元[1] 沈南[1] 薛峰[1] 裴军[1] 陈顺乐[1] 

机构地区：[1]上海第二医科大学附属仁济医院风湿病科,上海200001

出　　处：《中国免疫学杂志》1999年第10期477-479,共3页Chinese Journal of Immunology

基　　金：上海市科委自然科学基金

摘　　要：目的：初步探讨ＳＬＥ 患者周围血Ｔ细胞受体Ｖβ基因是否存在偏移。方法：应用荧光ＰＣＲ 定量方法对ＨＬＡＤＲＢ１＊ ０８０３／ＤＱＡ１ ＊ ０１０３ 的抗磷脂抗体阳性ＳＬＥ患者４ 例，病人对照干燥综合征（ＳＳ）１ 例，正常对照３ 例进行周围血ＴＣＲＶβ的分析。结果：健康者和病人对照的ＴＣＲ仅少数Ｖβ（ ＜５ 个Ｖβ基因） 有轻微增高表达。１ 例非活动性ＳＬＥ患者与对照组相仿，但３ 例活动性ＳＬＥ的ＴＣＲＶβ基因谱呈多克隆激活，其中２ 例有１５ ～１７ 个Ｖβ基因呈中度以至高度表达，甚至Ｖβ／Ｃα高达１００５％（Ｖβ４／Ｃα） 和２０８８ ％ （Ｖβ２／Ｃα） 。３ 例活动性ＳＬＥ的Ｖβ７、Ｖβ１２、Ｖβ２２ 都呈现异常增高表达，与正常对照有显著差异（ Ｐ ＜ ００５ ～００１）。结论：选择性的ＴＣＲＶβ基因的优势表达可能对ＳＬＥ的发病和活动起极大的作用。Objective:The preliminaryinguirnginto probable divergence TCR Vβusageofperipheralbloodlymphocytesin patients with SLE. Methods:4 patients with SLE,1 patientcontrolwith SS,accordingtothe Amenican college ofrheumatology criteria,3 normalcontrols were analyzedfor Tcell Vβrepertoire using fluorecent quantity PCRtechnique,all cases with positive antiphospholipid antibody and HLA DRB1* 0803DQA1 * 0103 .Results:Thethree healthy and one patient(SS) controlsshowed faintexpressionin only oneto five Vβfamilies respectively.The patient with non active SLEshowed asimilarresulttothe controls,whilethe otherthree,who had active disease,showed marked polyclonalactivityintheir Tcell Vβrepertoire,2 of3 affecting morethan 15 ofthe 23 Vβfamilies analyzed.Some Vβexpression was very high even Vβ4Cαand Vβ2Cαwith 100.5 % and 208.8 % respectively.3 cases of active SLEshowed marked abnormalexpression of TCRwith Vβ7,Vβ12, Vβ22 .There were significant different between patients with active SLEand controlsin TCR VβCα( P < 0.05 ～ 0 .01) .Conclusion:Dominontexpression ofselective TCR Vβwould play animportantrolein pathogenesis and disease activation ofSLE.

关 键 词：磷脂 抗体 系统性红斑狼疮 T细胞 受体 基因表达 

分 类 号：R593.241[医药卫生—内科学]