HIV-1 env与hIL-2基因在重组痘苗病毒中的共表达研究  

COEXPRESSION OF HIV 1 AND hIL 2 GENE IN RECOMBINANT VICCINIA VIRUS

作　　者：王玉红[1] 金宁一[1] 李体远[2] 王洪军 王弘郡 刘素寰 李萍[1] 安汝国[2] 殷震[1] 

机构地区：[1]解放军农牧大学研究所,长春130062 [2]白求恩医科大学细胞生物教研室,长春130021

出　　处：《免疫学杂志》1999年第4期217-219,共3页Immunological Journal

基　　金：中国杰出青年基金;自然科学基金

摘　　要：以痘苗病毒复制非必需区血凝素（ＨＡ）基因为侧翼，将编码人白细胞介素２（ｈＩＬ－２）的基因片段克隆到真核表达质粒ｐＪ３８ｅｎｖ 的下游，构建成含有ＨＩＶ－１ ｅｎｖ 和ｈＩＬ－２两种外源基因的重组表达质粒ｐＪ３８Ｅ－ＩＬ－２，经同源重组和血凝素阴性空斑筛选，获得了重组痘苗病毒ｖＪ３８Ｅ－ＩＬ２。经间接免疫荧光试验、Ｄｏｔ－ＥＬＩＳＡ和Ｗｅｓｔｅｒｎ ｂｏｌｔ等检测证明：重组病毒能同时表达Ｅｎｖ 蛋白和ＩＬ－２蛋白，表达产物分子量分别为９８×１０３ 、１６×１０３。With flank sequence of HA gene of vaccinia virus,coexpression plasmid pJ38E IL 2 containing cDNA of uman immunodeficiency virus type I envelope gene was constructed by inserting the hIL 2gene under the pJ38env plasmid vector.After homologous recombination in Cos 7 cell,were purified in BHK21 cell by using plaque assay.Indirect immunofluorescence assay(IFA)Dot EIISA and Western blot showed that the recombinant vaccinia virus had coexpressed the HIV 1 env and IL 2 protein in infected BHK21 and the molecular weight were about 98×10 3 and 16×10 3.

关 键 词：HIV-1 ENV HIL-2 痘苗病毒 艾滋病 

分 类 号：R512.910.1[医药卫生—内科学]