丙泊酚对大鼠肝微粒体细胞色素酶P450的影响  

Effect of propofol on liver microsomal cytochrome P450 in rats

作　　者：李振洲[1] 陈学新[2] 陈雅儒[1] 闫瑞[1] 孟尽海[2] 马汉祥[2] 邓丽琴[2] 

机构地区：[1]宁夏医科大学,银川市750004 [2]宁夏医科大学附属医院,银川市750004

出　　处：《实用医学杂志》2011年第3期393-395,共3页The Journal of Practical Medicine

基　　金：宁夏回族自治区自然科学基金项目(编号:NZ10114)

摘　　要：目的:研究丙泊酚对大鼠肝微粒体细胞色素酶P450含量的影响。方法:健康雄性SD大鼠18只,体重180～220g,随机分为苯巴比妥钠组、丙泊酚组、生理盐水组,每组6只,分别给予苯巴比妥钠75mg/kg,丙泊酚3.789mg/kg及等量生理盐水,持续3d。测定肝微粒体蛋白和P450的含量以及氨基比林-N脱甲基酶的活性。结果:与生理盐水组比较,苯巴比妥钠组、丙泊酚组肝微粒体蛋白和P450的含量升高;苯巴比妥钠组氨基比林-N脱甲基酶活性明显增高。结论:丙泊酚对大鼠肝微粒体蛋白、细胞色素P450具有诱导作用,对氨基比林-N脱甲基酶的活性无影响。Objective To explore the effect of propofol on liver microsomal cytochrome P450 in rats. Methods Eighteen male SD rats were randomly assigned to receive phenobarbital of 75 mg/kg （phenobarbital group, n = 6）, propofol of 3.789 mg/kg （propofol group, n = 6）, or normal saline （control group, n = 6） for three days. Levels of liver microsomal proteins and P450 were detected and activity of aminopyrine N- demethylase was determined by speetrophotometry. Results As compared with the control group, the levels of microsomal proteins and cytochrome P450 were increased in phenobarbital group and propofol group; the activity of aminopyrine N-demethylase was significantly elevated in phenobarbital group. Conclusions Propofol can induce liver microsomal cytochrome P450 in rats but has no effect on the activity of aminopyrine N-demethylase.

关 键 词：二异丙酚 苯巴比妥钠 P450 CYP2E1 

分 类 号：R965[医药卫生—药理学]