傅里叶变换红外光谱鉴别正常和良恶性乳腺组织  

FTIR Spectrum Comparisons Among the Breast tissues:the Normal Tissues,Hyperplasia,Fibroadenoma and Cancer

作　　者：赵瑾[1] 刘亚奇[1] 徐怡庄[2] 张元福[2] 吴瑾光[2] 侯宽永[1] 赵红梅[1] 雷玉涛[1] 周孝思[1] 徐智[1] 凌晓锋[1] 

机构地区：[1]北京大学第三医院普通外科,北京100191 [2]北京大学化学与分子工程学院,北京100871

出　　处：《高等学校化学学报》2011年第2期246-251,共6页Chemical Journal of Chinese Universities

基　　金：北京市自然科学基金项目(批准号:2072020)资助

摘　　要：采用傅里叶变换红外光谱(FTIR)及衰减全反射法(ATR)检测乳腺手术中切除的4种新鲜离体乳腺组织77例,包括正常乳腺组织18例,乳腺增生13例,乳腺纤维腺瘤21例及乳腺癌25例.用方差分析与秩和检验的方法比较了上述4类乳腺组织的红外光谱各谱带的峰位、相对峰强及半高宽.结果表明,与蛋白、脂质、糖类和核酸相关的谱带在上述4种不同类型乳腺组织之间均存在高度的统计学差异(P<0.05).因此,应用FTIR法可以区分正常乳腺组织、乳腺增生、乳腺纤维腺瘤以及乳腺癌等4种乳腺组织,这将为今后在手术中运用原位在体FTIR指导外科医生进行精准手术奠定基础.Fourier transform infrared（FTIR） spectra of 77 freshly removed breast tissues were collected by a spectrometer equipped with an Attenuated total reflectance（ATR） probe.These breast samples included 18 normal tissues,13 hyperplasia,21 fibroadenoma and 25 cancer.For each FTIR spectrum,the peak positions（P）,relative intensity ratios（I） and full width at half maximum（F） of the 13 bands were measured.The differences among the four types of breast tissue were compared by ANOVA（Analysis of Variance） and rank-sum test.These FTIR parameters were significantly different among the four types（P0.05）.In conclusion,FTIR spectra could illustrate the significant differences among the normal,hyperplasia,fibroadenoma and breast cancer tissues.It can present a novel approach of detecting cancer in situ and in vivo intraoperatively.

关 键 词：傅里叶变换红外光谱 乳腺癌 乳腺纤维腺瘤 乳腺增生 衰减全反射法 

分 类 号：O657.3[理学—分析化学]