Ag(Ⅰ)-呋塞米相互作用的共振瑞利散射、二级散射和倍频散射光谱及其分析应用  

Resonance Rayleigh Scattering,Second-order Scattering and Frequency Doubling Scattering Spectra of Interaction of Furosemide with Ag(Ⅰ) and Their Analytical Applications

作　　者：李翠侠[1] 刘绍璞[1] 刘忠芳[1] 胡小莉[1] 

机构地区：[1]西南大学化学化工学院,发光与实时分析教育部重点实验室,重庆400715

出　　处：《高等学校化学学报》2011年第2期252-257,共6页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20875078);发光与实时分析重庆市重点实验室基金(批准号:2006CA8006)资助

摘　　要：在pH=3.5的HAc-NaAc介质中,呋塞米(FUR)与Ag(Ⅰ)形成1∶1(摩尔比)的螯合物,从而引起共振瑞利散射(RRS)、二级散射(SOS)和倍频散射(FDS)光谱显著增强,其最大RRS,SOS和FDS波长分别位于310,584和330 nm.在一定范围内,3种散射信号的增强(ΔIRRS,ΔISOS和ΔIFDS)均与呋塞米浓度成正比.该方法具有较高灵敏度,RRS,SOS和FDS法对FUR的检出限(3σ)分别为1.3,3.0和6.9 ng/mL.考察了适宜的反应条件和共存物质的影响,结果表明方法具有良好的选择性.据此,提出了简便、快速、准确且高灵敏测定痕量呋塞米的光散射新方法,并应用于片剂和尿样中呋塞米的测定.同时对反应机理和散射光谱增强的原因进行了讨论.In a HAc-NaAc buffer solution（pH=3.5）,furosemide reacted with Ag（Ⅰ） to form a 1∶1（molar ratio） chelate.In this case,the intensities of resonance Rayleigh scattering（RRS）,second-order scattering（SOS） and frequency doubling scattering（FDS） spectra were enhanced greatly and the new scattering spectra appeared.The maximum RRS,SOS and FDS wavelength was located at 310,584 and 330 nm,respectively.The intensities of RRS,SOS and FDS were directly proportional to the concentration of furosemide in a certain range and the detection limits were 1.3,3.0 and 6.9 ng/mL,respectively.In addition,the optimum conditions and the influences of coexisting substances were tested by RRS method and the results show that this method has good selectivity.Therefore,a new simple,rapid,and sensitive light scattering method has been developed for the determination of furosemide in tablets and urine samples.In addition,the reaction mechanism and the reasons for the enhancement of scattering spectra were discussed.

关 键 词：呋塞米 Ag(Ⅰ) 共振瑞利散射光谱 二级散射 倍频散射 

分 类 号：O657.3[理学—分析化学]