醇/盐双水相体系中α-环己基扁桃酸手性分子的识别  

Chiral Recognition of α-Cyclohexyl-mandelic Acid in Alcohol/Salt-based Aqueous Two-phase Systems

作　　者：郭志峰[1] 李芬芳[1] 邢健敏[1] 

机构地区：[1]中南大学化学化工学院,长沙410083

出　　处：《高等学校化学学报》2011年第2期275-280,共6页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20956001);湖南省自然科学重点基金(批准号:08jj3023)资助

摘　　要：以含羟丙基-β-环糊精(HP-β-CD)手性识别剂的醇/盐双水相体系作为一种新型的手性识别萃取体系,研究了α-环己基扁桃酸(CHMA)对映体在其中的手性识别行为.考察了HP-β-CD浓度、CHMA浓度、乙醇和硫酸铵质量分数、体系温度和pH值等因素对CHMA对映体分配比(D)和分离因子(α)的影响.结果表明,含有手性识别剂HP-β-CD的乙醇/硫酸铵双水相体系对CHMA对映体具有很强的手性识别能力;体系中HP-β-CD浓度、乙醇质量分数、温度和pH值等因素对对映体的分离度影响较大;在体系温度为40℃、pH值为2、乙醇质量分数为30%、硫酸铵质量分数为15%、HP-β-CD的浓度为50 g/L及CHMA浓度为0.5 mmol/L时,手性识别分离效果最佳,分离因子α达到1.86.A novel alcohol/salt-based aqueous two-phase system containing chiral selector hydroxypropyl-β-cyclodextrin（HP-β-CD） for enantioselective liquid-liquid extraction was introduced.The chiral recognition of α-cyclohexyl-mandelic acid（CHMA） enantiomers was studied in this system.The effect factors of the distribution ratio（D） and the separation factors（α） on parameters such as the concentration of HP-β-CD,the concentration of CHMA,the mass fractions of C2H5OH and（NH4）2SO4,temperature and pH have been investigated.It was found that the C2H5OH-（NH4）2SO4-H2O aqueous two-phase system containing HP-β-CD as chiral selector showed a good chiral recognition ability toward CHMA enantiomers.It could be concluded that the enantioseparation strongly depended on the concentration of HP-β-CD,the mass fraction of C2H5OH,temperature and pH.The optimum conditions of enantioseparation were as follows： the temperature was 40 ℃,the pH was 2.0,the mass fractions of C2H5OH and（NH4）2SO4 were 30% and 15%,respectively,the concentration of HP-β-CD was 50 g/L,and the concentration of CHMA was 0.5 mmol/L.Under these conditions the separation factor（α） reached to 1.86.

关 键 词：双水相体系 手性识别 α-环己基扁桃酸对映体 羟丙基-Β-环糊精 

分 类 号：O652.63[理学—分析化学] O621.14[理学—化学]