法舒地尔对肺血管重构的肺动脉高压大鼠的影响  

Effects of fasudil on pulmonary arterial hypertension with vascular remodeling in rats

作　　者：左万里[1] 黄积雄[1] 黄炎明[1] 周伟[2] 程道胜[3] 仝金斋[1] 

机构地区：[1]中山大学附属江门医院,江门市中心医院呼吸内科,江门市呼吸疾病研究所,广东江门529000 [2]中山大学附属江门医院,江门市中心医院病理科,广东江门529000 [3]中山大学附属江门医院,江门市中心医院输血科,广东江门529000

出　　处：《中华实验外科杂志》2011年第2期271-273,F0003,共4页Chinese Journal of Experimental Surgery

摘　　要：目的 观察法舒地尔对发生肺血管重构的肺动脉高压（PAH）大鼠的影响.方法 将32只雄性Wistar大鼠随机均分为4组：对照3周组,模型3、6周组及治疗组;予野百合碱建模;治疗组于3周后予法舒地尔腹腔内注射.于3、6周末测定各组大鼠右心室收缩压（RVSP）、内皮素-1（ET-1）、N端脑钠素前体（NT-proBNP）、肺小动脉病理等.结果 （1）造模后RVSP随时间升高（31.2±7.6）、（43.3±8.3）、（56.9±6.9）mm Hg（1 mm Hg=0.133 kPa）,治疗组（47.3±6.3）mmHg较模型6周组明显降低.（2）ET-1、NT-proBNP与RVSP呈正相关（r=0.721、0.454）.（3）病理：造模后肺动脉平滑肌增生,管壁狭窄,至6周时近于闭塞,治疗组则明显好转.结论 对于已发生肺血管重构的PAH大鼠,法舒地尔能阻止肺动脉高压进展,ET-1、NT-proBNP可成为较理想的评估指标.Objective To investigate the effects of Rho kinase inhibitor fasudil on monocrotaline （MCT）-induced pulmonary arterial hypertension （PAH） with vascular remodeling in rats. Methods Thirty-two male Wistar rats were randomly and equally divided into control group （ C3 ）, MCT-PAH 3- and 6-week model groups （H3, H6 ） and fasudil-treated group （F6）. The right ventricle systolic pressure（ RVSP）, right ventricle/left ventricle and septum[RV/（ LV ＋ S）], plasma endothelin-1 and NT-proBNP were measured; small pulmonary arterial morphometry, ratio of vascular wall thickness to vascular external diameter （WT） and ratio of vascular wall area to the total area （WA） were also observed. Results （ 1 ）The RVSP was highest in H6 group, followed by F6 group, H3 group, and C3 group[（56. 9 ±6. 9）, （47. 3±6.3）, （43.3±8.3） and （31.2±7.6） mm Hg （1 mm Hg=0. 133 kPa）]. But no significant difference was found between H3 and F6 groups; （2） The levels of plasma ET-1 and NT-proBNP were positively associated with RVSP, RV/（LV ＋S）, WT and WA （r for ET-1 was 0.721, 0.607, 0.652, 0.707,P〈0.01; r for NT-proBNP was 0. 454, 0. 330, 0. 365, 0. 398,P 〈0. 05）; （3） Pathematological assessment showed that the thickness and muscularization of small pulmonary artery were increased in H3 group as compared with C3 group, most significantly in H6 group, and those were significantly decreased in F6 group as compared with H6 group. Conclusion Fasudil can improve the MCT-induced PAH with vascular remodeling in rats. The plasma ET-1 and NT-proBNP could be used as good makers to evaluate the severity of PAH.

关 键 词：肺动脉高压 法舒地尔 内皮素-1 

分 类 号：R544.16[医药卫生—心血管疾病]