资阳香橙×枳杂种苗鉴定及耐碱株系筛选  

Preliminary identification of alkali-resistance hybrid seedlings of Junos and Trifoliate orange

作　　者：曹立[1,2] 彭良志[2] 彭爱红[2] 凌丽俐[2] 江东[1,2] 王福生[1] 范达[1] 王军政[1] 马小焕[1] 张雯雯[1] 常青华[1] 王小雪[3] 

机构地区：[1]西南大学园艺园林学院,重庆400715 [2]西南大学柑橘研究所,重庆400715 [3]西南大学资源环境学院,重庆400715

出　　处：《果树学报》2011年第1期20-25,共6页Journal of Fruit Science

基　　金：国家科技支撑计划项目(2007BAD47B042007BAD61B05);公益性行业(农业)科研专项(nyhyzx07-023);国家现代农业(柑橘)产业技术体系建设专项;重庆市科委重大专项(CSTC2007AA1016);重庆市自然科学基金(CSTC2009BB1137)

摘　　要：为培育耐碱的杂种砧木,以耐碱品种资阳香橙(Citrus junos Sieb.ex Tanaka)与不耐碱的枳[Poncirus trifoliata(L.)Raf.]杂交,将杂种F1群体经形态学与SSR鉴定杂种真实性,并通过叶片黄化程度分级、叶绿素与有效铁质量分数的测定以筛选耐碱杂种单株。结果表明,(1)与母本资阳香橙叶片形态上无明显差异的F1都是珠心苗。(2)真实杂种苗从第10片叶开始都有三出复叶。(3)筛选到2对SSR引物能鉴定出全部杂种苗。(4)在碱性土上,耐碱杂种苗叶绿素cha+b的质量分数显著低于耐碱亲本资阳香橙,而极显著高于不耐碱亲本枳;但叶绿素a的质量分数显著高于父母本。5、耐碱杂种叶片中有效铁质量分数显著高于枳。研究获得了2株耐碱能力较强的真实杂种苗。In order to obtain high tolerance rootstocks to calcareous soil from citrus inter-genera offspring,hybridization were made with artificial cross-pollination method between Ziyangxiangcheng（Citrus junos Sieb.ex Tanaka） and Trifoliate orange [Poncirus trifoliata（L.） Raf.].The zygotic offspring were identified both by visible morphological characteristics and SSR markers.After growing on calcareous soil for one year,the tolerance capability of zygotic offspring was evaluated based on the degree of leaf yellowing from no yellowing symptom to extreme yellowing.The contents of chlorophyll and active iron in leaves were also analyzed.Two SSR primers were selected to identify all zygotic offspring.The result indicated that the first trifoliate leaflet usually appeared on the second leaf bud to the tenth leaf bud of the true zygotic offspring.After growing on the alkali soil of pH 8.1 for 2 years,the total contents of leaf chlorophyll a and b of the tolerance hybrids were significantly lower than Ziyangxiangcheng but significantly higher than trifoliate orange.At the same time the contents of chal a were higher than both parents.Meanwhile the contents of active iron in leaves of the tolerant hybrids were higher than trifoliate orange.Two hybrid seedlings with high tolerance to alkali soil were screened out by far.

关 键 词：柑橘 砧木 杂种鉴定 耐碱 SSR鉴定 

分 类 号：S666[农业科学—果树学]