石榴种质资源遗传多样性及亲缘关系的ISSR分析  

Genetic diversity and genetic relationship of pomegranate (Punica granatum) germplasm evaluated by ISSR markers

作　　者：赵丽华[1] 李名扬[2] 王先磊[2] 刘华敏[3] 

机构地区：[1]西昌学院农业科学学院,四川西昌615013 [2]西南大学园艺园林学院,重庆市花卉工程技术研究中心,重庆400715 [3]重庆市园林绿化科学研究所,重庆400715

出　　处：《果树学报》2011年第1期66-71,共6页Journal of Fruit Science

基　　金：四川省教育厅科研基金(08zc014);西昌学院课题(YJSA0615)

摘　　要：利用ISSR标记技术对47个石榴品种遗传关系进行了分析。筛选出多态性高的6条ISSR引物,共扩增出120条DNA条带,其中多态性带109条,多态性百分率为90.83%,有效等位基因数(Ne)、Nei’s基因多样(H)、Shan-non信息指数(I)分别为1.294 5±0.309 4、0.189 7±0.161 8、0.309 1±0.219 8,遗传距离(Dg)变异为0.075 0～0.400 0,表明石榴品种间存在比较丰富的遗传多样性。利用UPGMA法构建分子树状图,将47个石榴品种分为5个类群。同时检测到15条特异性条带,可用于供试石榴中的11个品种鉴定的参考性标记。ISSR markers was used to analyse the genetic relationship of 47 pomegranate cultivars.6 polymorphic primers were used for PCR,and amplied 120 bands in which 109 bands is polymorphic.The percent of polymorphic is 90.83%.The effective number of alleles（Ne）,gene diversity of Nei（H）,information index of Shannon（I） were 1.294 5±0.309 4,0.189 7±0.161 8,0.309 1±0.219 8 respectively.Range of genetic distance（Dg） is from 0.075 0 to 0.400 0.The results showed higher level of genetic diversity among of pomegranate cultivars.Based on UPGMA,the 47 pomegranate cultivars were divided into 5 groups.15 special bands were detected,which may be used as reference of 11 pomegranate cultivars identity.

关 键 词：石榴 ISSR 种质资源 遗传多样性 亲缘关系 

分 类 号：S665.4[农业科学—果树学]