二次回归正交旋转组合设计在梨子叶不定梢再生研究中的应用  

Application of quadratic regressive orthogonal rotational combinational design for regeneration study of pear cotyledon adventitious shoots

作　　者：鲁敏[1] 汤浩茹[1] 余世杰[1] 雷美艳[1] 陈小杨[1] 丁继军[1] 

机构地区：[1]四川农业大学园艺学院,四川雅安625014

出　　处：《果树学报》2011年第1期171-175,共5页Journal of Fruit Science

摘　　要：以水晶×金二十世纪花后90 d的梨成熟胚子叶为外植体,利用二次回归正交旋转组合设计对其再生体系研究中的关键因素6-BA、NAA、AgNO3浓度建立数学模型,得到回归方程Y=4.405 3+0.863 8X1-0.386 9X2-0.058 4X3-0.015 7X1X2+0.000 5X1X3+0.011 2X2X3-0.671 8X12-0.219 2X22-0.749 9X32。6-BA、NAA、AgNO3浓度在试验设定范围内对梨成熟胚子叶不定梢再生系数的影响均呈"低促高抑"现象;且培养基中6-BA、NAA、AgNO3的浓度分别在5.955～7.124 mg·L-1、0.281～0.473 mg·L-1和2.199～2.801 mg·L-1,梨成熟胚子叶不定梢再生系数大于3的可靠性为95%。Cotyledons of seeds from the cross Sujeong×Gold-Nijisseiki were taken as explants on 90 d after pollination.Mathematical model was constructed with 6-BA,NAA and AgNO3 using quadratic regressive orthogonal rotational combinational design,Y=4.405 3＋0.863 8X1-0.386 9X2-0.058 4X3-0.015 7 X1X2＋0.000 5X1X3＋0.011 2X2X3-0.671 8X12-0.219 2X22-0.749 9X32.The results showed that the regeneration coefficient was higher when the concentration of 6-BA,NAA and AgNO3 were lower;and that the coefficient was more than 3 with the probability of 95% when 6-BA concentration was in a range of 5.955 to 7.124 mg·L-1,NAA in 0.281 to 0.473 mg·L-1 and AgNO3 in 2.199 to 2.801 mg·L-1.

关 键 词：梨 子叶 二次回归正交旋转组合设计 再生体系 数学模型 

分 类 号：S661.2[农业科学—果树学]