延衰合剂对D-半乳糖衰老小鼠血清SOD、MDA、IL-2和睾酮的影响  

Effects of Yanshuai Mixture on Serum SOD, MDA, IL-2 and Testosterone of Aging Mice Induced by D-galactose

作　　者：栾增强[1] 曹文富[1] 黄和贤[1] 李志强[1] 

机构地区：[1]重庆医科大学附属第一医院中西医结合科,重庆市400016

出　　处：《中国药房》2011年第7期589-591,共3页China Pharmacy

摘　　要：目的:研究延衰合剂(Yanshuai Mixture,YSM)对D-半乳糖诱导的亚急性衰老模型小鼠血清抗氧化指标、白细胞介素-2(IL-2)和睾酮(TS)水平的影响。方法:60只小鼠随机分为正常对照(N)、模型(M)、补肾益寿胶囊(BSYS)和YSM高、中、低剂量(YSM-H、YSM-M、YSM-L)组,用D-半乳糖连续皮下注射复制衰老小鼠模型,同时给予YSM连续灌胃8周。测定血清总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量;酶联免疫吸附法(ELISA)检测血清IL-2和TS的变化。结果:与N组比较,M组小鼠血清T-AOC、SOD活性显著降低,MDA含量显著增加,血清IL-2、TS水平显著降低(P<0.05);与M组比较,YSM-H组小鼠血清T-AOC、SOD显著增加,MDA含量显著减少(P<0.05);YSM各剂量组血清IL-2、TS水平显著增加(P<0.05)。结论:YSM可以显著提高D-半乳糖衰老小鼠血清抗氧化能力,抑制IL-2和TS水平的下降,可能具有良好的延缓衰老的作用。OBJECTIVE： To study the effects of Yanshuai mixture （YSM） on serum antioxidant index, interleukin-2 （IL-2） and testosterone （TS） levels of D-galactose induced subacute aging mice. METHODS： 60 mice were randomly divided into normal control group （N）, aging model group （M）, Bushen yishou capsule group （BSYS）,YSM low （YSM-L）, medium （YSM-M） and high （YSM-H） dose groups. The subacute aging model mice were induced by injecting with D-galactose subcutaneously. At the same time, YSM were administered intragastrically （i.g.） for 8 weeks. Total antioxidant capacity （T-AOC）, superoxide dismutase （SOD） activity, malondialdehyde （MDA） content in serum were measured; IL-2 and TS levels in serum were detected by ELISA. RESULTS： Compared with normal control group, the levels of T-AOC and SOD in serum of aging model group were significantly decreased, while MDA content increased significantly, and levels of IL-2 and TS were significantly reduced （P〈0.05）. The levels of T-AOC and SOD in serum of YSM high-dose group of mice was increased significantly, while MDA content decreased signifi- cantly, the levels of IL-2 and TS in serum of each YSM dose groups were increased significantly, compared with aging model group （P〈0.05）. CONCLUSION： YSM can significantly improve serum antioxidant capacity, inhibit the decline of IL-2 and TS levels in D-galactose induced aging mice, may has good anti-aging effect.

关 键 词：延衰合剂 衰老 抗氧化 白细胞介素-2 睾酮 

分 类 号：R285.5[医药卫生—中药学] R97[医药卫生—中医学]