抑肽酶对实验性慢性肝损伤大鼠肝细胞增殖能力的影响  

Influence of aprotinin in hepatocyte proliferation ability in rats with experimental chronic liver injury

作　　者：王虹蛟 王心童 王强 孟威宏[3] 颜炜群[4] 任立群[4] 

机构地区：[1]解放军第461医院内科,吉林长春130021 [2]吉林大学白求恩医学院 [3]沈阳军区总医院心血管内科,辽宁沈阳110015 [4]吉林大学再生医学科学研究所,吉林长春130021

出　　处：《吉林大学学报（医学版）》2011年第1期18-20,I0001,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30300153)

摘　　要：目的:探讨抑肽酶对实验性慢性肝损伤大鼠肝细胞增殖能力的影响,为抑肽酶对慢性肝损伤的保护作用研究提供理论依据。方法:Wistar大鼠随机分为正常对照组、四氯化碳(CCl4)模型组、抑肽酶小剂量组、抑肽酶中剂量组、抑肽酶大剂量组和促肝细胞生长素组,采用免疫组织化学方法检测各组增殖细胞核抗原(PCNA)阳性细胞数和形态学表现。结果:正常对照组阳性细胞数为(4±2)个/视野,模型组阳性细胞数为(5±2)个/视野,抑肽酶小、中和大剂量组阳性细胞个数分别为(39±13)个/视野、(49±14)个/视野和(57±12)个/视野,促肝细胞生长素组中阳性细胞个数为(26±8)个/视野。抑肽酶各剂量组与模型组相比,阳性细胞数目均显著增加(P<0.05);随着抑肽酶剂量的增加,阳性细胞数目也增加。抑肽酶各剂量组阳性细胞数多于促肝细胞生长素组(P<0.05)。抑肽酶各剂量组PCNA阳性细胞增殖活跃,以抑肽酶大剂量组表现明显。结论:抑肽酶能够促进慢性肝损伤大鼠肝细胞增殖,其作用随着剂量的增加而增强。Objective To study the influence of aprotinin in hepatocyte proliferation ability in rats with experimental chronic liver injury and provide thoreotical basis for study on protective effect of aprotinin on chronic liver injury.Methods Wistar rats were randomly divided into normal control group,carbon tetrachloride（CCl4） model group,low dose aprotinin group,midde dose aprotinin group,high dose aprotinin group and hepatocyte group.Immunohistochemical method was used to observe the proliferating cell nuclear antigen（PCNA）-positive cell number in various groups.Results The positive cell number in control group was（4±2）/vision;（5±2）/vision in model group;（39±13）/vision,（49±14）/vision and（57±12）/vision in low,middle,and high dose groups;（26±8）/vision in hepatocyte group.The positive cell numbers in aprotinin groups were markedly increased compared with model group（P0.05）,the positive cell number was increased with the increase of aprotinin dose.The positive cell numbers in aprotinin groups were more than that in hepatocyte group（P0.05）.The proliferation of PCNA positive cells in each aprotinin group was significantly increased,especially in high dose aprotinin group.Conclusion The aprotinin could promote the proliferation of chronicly injured liver cells and this effect could be enhanced by the increasing of aprotinin dose.

关 键 词：抑肽酶 四氯化碳 慢性肝损伤 大鼠 Wistar 免疫组织化学 细胞增殖 

分 类 号：R575[医药卫生—消化系统]