雄激素受体基因E211 G＞A多态性与汉族女性青春期后痤疮的关联性分析  

Relevance analysis on E211 G>A androgen receptor gene polymorphism and female postadolescent acne in Han population

作　　者：田黎明[1] 谢红付[1] 杨婷[2] 王玮蓁[3] 李吉[1] 

机构地区：[1]中南大学湘雅医院皮肤科,湖南长沙410008 [2]昆明医学院第一附属医院皮肤科,云南昆明650032 [3]华中科技大学武汉第一医院皮肤科,湖北武汉430022

出　　处：《吉林大学学报（医学版）》2011年第1期113-116,共4页Journal of Jilin University：Medicine Edition

基　　金：湖南省卫生厅科研基金资助课题(2006-238)

摘　　要：目的:探讨雄激素受体(AR)基因E211G>A单核苷酸多态性与汉族女性青春期后痤疮的关联性,为汉族女性青春期后痤疮发病遗传学研究奠定基础。方法:将女性青春期后痤疮患者79例和正常女性志愿者80例分为女性青春期后痤疮组和女性正常对照组,采用PCR技术扩增出AR-E211G>A基因多态位点的片段,用限制性内切酶StuⅠ进行酶切,产物在2%琼脂糖凝胶上电泳,确定G>A基因的3种基因型,即GG、GA、AA,观察两组基因型频数和等位基因型频数分布的差异。结果:女性青春期后痤疮组GA+AA基因型频数低于对照组(P=0.028,OR=0.937,95%CI=0.885～0.992)。女性青春期后痤疮组A等位基因频数低于对照组(P=0.029,OR=0.968,95%CI=0.941～0.996)。结论:AR-E211A等位基因的存在使青春期后女性患痤疮的风险性明显降低。Objective To explore the correlation between the E211 G>A androgen receptor(AR) gene polymorphism and female postadolescent acne of Han Chinese,and lay the foundation for genetic studies of female postadolescent acne of Han Chinese.Methods The female postadolescent acne patients(n=79) and the healthy people(n=80) were individed into female postadolescent acne group and control group.The polymorphism was investigated by PCR using DNA from peripheral blood lymphocytes.The transition(G>A) in the risk allele(A) produced a new recognition site for the restriction endonuclease StuⅠ.Three genotypes of AR-E211 G>A gene(GG,GA,AA) were determined,and the differences in the frequencies of the genotype and the allele between female postadolescent acne group and control group were observed.Results There was a significant difference in the frequency of the genotype GA+AA in the AR-E211 genetic polymorphisms between female postadolescent acne patients and healthy control(P=0.028,OR=0.937,OR 95% CI=0.885-0.992);and there was also a significant difference in the allele(A) frequency between female postadolescent acne patients and healthy control(P=-0.029,OR=0.968,OR 95% CI=0.941-0.996).Conclusion The high frequency of A allele in the functional G1733A polymorphism of AR-E211 is a decreased risk factor for female postadolescent acnein Han Chinese.

关 键 词：雄激素受体 基因多态性 女性青春期后痤疮 汉族 遗传学 

分 类 号：R758.73[医药卫生—皮肤病学与性病学] R349.64[医药卫生—临床医学]