枸杞体细胞胚发生中蛋白质和DNA代谢动态的立体计量  

QUANTITATIVE STEREOLOGIC ANALYSIS OF METABOLIZE DYNAMIC OF PROTEIN AND DNA DURING SOMATIC EMBRYOGENSIS IN LYCIUM BARBARUM L.

作　　者：戴若兰[1] 张玮[1] 邢更生[1] 崔凯荣[1] 王亚馥[1] 

机构地区：[1]兰州大学干旱农业生态国家重点实验室,兰州730000

出　　处：《实验生物学报》1999年第3期296-300,共5页Acta Biologiae Experimentalis Sinica

基　　金：国家自然科学基金(批准号:39770375);甘肃省自然科学基金(批准号:ZR-95-012)

摘　　要：本文以宁夏构杞无菌苗叶片为材料,离体培养,并诱导体细胞胚发生。根据细胞形态计量学原理,应用数字图像处理软件计量由光学底片经A/D转换成的数字图像中的蛋白质和DNA大分子,对于构杞体细胞胚发生过程中蛋白质和DNA分子的代谢动态进行了量化处理,并对量化结果分析了蛋白质和DNA代谢动态与体细胞胚发生、发育的关系。The protein and DNA content in somatic embryos of Lycium barbarum L were analysed using quantitative stereologicai procedure formed from Cell Morphometry. The photos were transformed into digital images by the Sharp JX-610 scanner. Then the quantity of protein and DNA was count applying digital images processing software. By the result we discuss the relationship between changes in protein and DNA metabolize dynamic and somatic embryogensis.

关 键 词：枸杞 体细胞胚 蛋白 DNA 代谢 立体计量 

分 类 号：S567.19[农业科学—中草药栽培]