猕猴小肠5-羟色胺内分泌细胞的分布及形态学研究  

Distribution and Morphology of the 5HT Endocrine Cells in the Small Intestine of Rhesus Monkeys

作　　者：张顺利[1] 张新胜[1] 张文学[1] 郭玫[1] 杨向莹 刘清兰[1] 

机构地区：[1]河南师范大学生物系,新乡453002

出　　处：《上海实验动物科学》1999年第3期133-136,共4页Shanghai Laboratory Animal Science

基　　金：河南省动物学省级重点学科资助项目

摘　　要：以免疫组织化学链霉卵白素（ Ｓ Ｐ）法显示猕猴小肠５ 羟色胺（５ Ｈ Ｔ）内分泌细胞，并用 Ｗｅｉｂｅｌ体视学方法对其进行了定量分析。结果表明，此种细胞的密度在十二指肠最高，空肠密度中等，回肠密度最低；５ Ｈ Ｔ 免疫反应阳性细胞多分布在肠腺处，绒毛中较少。该细胞形态多样，大多为锥形、梭形、椭圆形或圆形。有的细胞基底部有突起，后者可伸向腺腔或肠腔，其末端含有５ Ｈ Ｔ 阳性物质。Six rhesus monkeys were used to study the distribution and morphology of 5hydroxytryptamine (5HT) endocrine cells in the small intestine by using immunohistochemical streptavidin/peroxidase (SP) method,and Weibel's stereological method.The results were as follows:① the density of 5HT endocrine cells in the small intestine of the rhesus monkeys was the highest in the duodenum and moderate in the jejunum and the lowest in the ileum;② 5HT endocrine cells were distributed mainly in the glandulate duodenales and less in the villi;③ 5HT cells were various in shape,mostly oval,shuttle,pyramidal or round;and ④ basal portion of some endocrine cells had processes,extended to luminal surface of the crypt or intestinal tract with 5HT positive substance accumulating in their ends.These results suggested that 5HT endocrine cells have paracrine and exocrine functions.

关 键 词：猕猴 小肠 血清素 内分泌细胞 免疫组化 

分 类 号：Q959.848[生物学—动物学]