奶牛胚胎移植中GnRH、PGF_(2α)同期发情处理效果的比较  

COMPARISON BETWEEN GnRH AND PGF 2α ON ESTRUS SYNCHRONIZATION IN DAIRY COWS

作　　者：和协超[1] 纪少珲[1] 张僖 和占星 文际坤 季维智[1] 

机构地区：[1]中国科学院昆明动物研究所,昆明650223 [2]昆明市农场管理局,昆明650034 [3]云南牧草和肉牛研究中心,昆明650212

出　　处：《Zoological Research》1999年第5期332-335,共4页动物学研究（英文）

基　　金：云南省重点基金项目;中国科学院重点资助

摘　　要：同期发情处理（ｅｓｔｒｕｓｓｙｃｈｒｏｎｉｚａｔｉｏｎ） 是牛胚胎移植中一项重要的技术，不同的方法因不同的环境条件产生不同的效果。本文就同期发情的不同方法进行了比较，以寻找适合当地条件的同期发情方案。１９３ 头健康、经产的黑白花奶牛分为３ 个组，用３ 种不同的方法进行同期发情处理和胚胎移植。第１ 组：注射１ 次Ｌｕｔａｌｙｓｅ；第２ 组：注射２ 次Ｌｕｔａｌｙｓｅ；第３ 组：注射ＦｅｒｔａｇｙｌＬｕｔａｌｙｓｅＦｅｒｔａｇｙｌ。结果表明：第１ 组和第２ 组之间的同期发情胚胎移植率无显著差异（ Ｐ＞ ０－０５） ，分别为２６－５％ （２１／７９）和２７－０％ （１８／６８）；第３ 组的同期发情胚胎移植率为６０－９ ％（２８／４６） ，显著高于第１ 组和第２ 组（ Ｐ＜ ０－０５）。结果提示ＦｅｒｔａｇｙｌＬｕｔａｌｙｓｅＦｅｒｔａｇｙｌ 可能是较适合黑白花奶牛的同期发情处理方案。Estrus synchronization is an important technique in embryo transfer.Several methods have been used in cow synchronization.However,previous reports showed that the efficiency of synchronization would be different due to the situation of the recipients as well as other conditions.In the present study,the effect of GnRH on estrus synchronization of cow recipients in embryo transfer was determined,and compared with that of using PGF 2α method.193 hostein cows reared in the Kunming farm were randomly divided into 3 groups.Group 1,recipients were administered once 25?mg PGF 2α (5?mg/mL).Group 2,cows were injected PGF 2α twice,with 11 day interval between two administration,25?mg/one injection.Group 3,cows were injected GnRH (86?μg) followed by injection of 25?mg PGF 2α 7 days later and a second GnRH (86?μg) administration 16 days later.Results showed that in group 3 there were more transferable recipients (60 9%) for embryo transfer compared with that in group 1 (26 5%) and in group 2 (27 8%),respectively.It seems that interval injection of GnRH/PG/GnRH is a better method of estrous synchronization.

关 键 词：奶牛 同期发情 前列腺素 GNRH 胚胎移植 

分 类 号：S823.91[农业科学—畜牧学] S823.36[农业科学—畜牧兽医]