活性氧对苏云金芽孢杆菌伴孢晶体的损伤作用  

THE EFFECTS OF REACTIVE OXIDANTS ON BACILLUS THURINGIENSIS PARASPORAL CRYSTALS

作　　者：王文军[1] 钱传范[1] 申继忠[1] 杨韶松 

机构地区：[1]中国农业大学应用化学系,北京100094

出　　处：《微生物学报》1999年第5期469-474,共6页Acta Microbiologica Sinica

摘　　要：用ＳＤＳＰＡＧＥ 电泳分析和生物测定方法研究了过氧化氢（Ｈ２Ｏ２） 和羟自由基（·ＯＨ）对苏云金芽孢杆菌（ Ｂａｃｉｌｌｕｓ ｔｈｕｒｉｎｇｉｅｎｓｉｓ） 伴孢晶体的损伤作用。结果表明，这两种活性氧对伴孢晶体均有一定程度的损伤作用，这种损伤作用与活性氧的浓度成正相关，并且·ＯＨ 对伴孢晶体的损伤作用明显强于Ｈ２Ｏ２ 。The effects of hydrogen peroxides(H2O2)and hydroxyl radicals(·OH)on the purified parasporalcrystalsfrom Bacillusthuringiensisvar. Kurstaki HD 1 werestudiedby SDS PAGEand bioassay.Theresultsshowed thatto some extent H2O2 and ·OHproduced a direct and deleterious effect. Hydroxylradicalsfrom the system Fe( Ⅱ) EDTA H2O2indicated more serious damagethan hydrogen peroxides. There was a 44-5 % loss in activity against 12 h old larvae of Heliothis armigera when parasporalcrystals weretreated with 0-3 % H2O2 only. However,85-2 % activity lost when parasporalcrystals weretreated with 0-3% H2O2in thepresenceofiron( Ⅱ) and EDTA.

关 键 词：过氧化氢 羟自由基 苏云金芽孢杆菌 伴孢晶体 

分 类 号：Q939.124[生物学—微生物学]