应用染色体涂染法建立人和黑叶猴(Semnopithecus francoisi)的染色体同源性  

Chromosome Homologies Between Human and Francois' Monkey(Semnopithecus francoisi) Established by Chromosome Painting

作　　者：佴文惠[1] 刘瑞清[1] 陈玉泽[1] 王金焕[1] 

机构地区：[1]中国科学院昆明动物研究所,昆明650223

出　　处：《Acta Genetica Sinica》1999年第5期474-479,共6页

基　　金：云南省应用基础研究基金

摘　　要：应用荧光原位杂交技术中的染色体涂染法（Chromosomepainting），以生物素标记的除Y染色体外的人全部整条染色体DNA特异性探针与黑叶猴的中期分裂相杂交，建立了人与黑叶猴之间的染色体同源性。除人的1、2、6、16和19号染色体特异探针分别与黑叶猴的2条非同源的染色体杂交外，其余人染色体特异探针均与黑叶猴的1条染色体杂交，其中有两对人染色体特异探针（14和15，21和22）分别杂交同一条黑叶猴染色体。在雌性黑叶猴的单倍染色体中，共检测到30个与人染色体具同源性的染色体和染色体片段。结果表明：黑叶猴的多数染色体与人染色体有高度同源性，仅有少数染色体发生了重排。将研究的结果与已报道的人染色体特异探针与其他灵长类的中期染色体杂交的结果进行比较，可以看出亚洲叶猴之间的相互关系较与非洲叶猴的更为密切。Chromosomal homologies were established between human and Francois' monkey(Semnopithecus francoisi, 2n = 44) by chromsome Painting with chromosome-specificDNA probes of all human chromosoms except the Y. Except for human chromosome 1,2, 6, 16 and 19 probes which gave signals on two nonhomologous S francoisichromosomes respectively, all other probes each hybridized to a single chromosome. Onlytwo S francoisi chromosomes (No. 12 and No. 21) were each labelled by two separateprobes (14 and 15, 21 and 22, respechvely). In total, 23 human chromosome-specificprobed detected 30 homologous chromosomes and chromosomal segments in the haploid Sfrancoisi genome. The results indicated a high degree of conservation of chromosomalsynteny between human and this langur. Only some chromosomal rearrangements occurredin this langur. Comparison of the hybridization patterns of human painting probes on thislangur with the data on other primates suggested the Asian langurs were karyotypicallymore closely related to each other than to African langurs.

关 键 词：黑叶猴 人 染色体同源性 染色体涂染法 

分 类 号：Q953.3[生物学—动物学] Q349.1