转BADH基因烟草的光系统Ⅱ和呼吸酶活性变化  

Changes of Photosystem Ⅱ and Respiratory Enzyme Activity in Transgenic Tobacco Enriched BADH Gene 

作　　者：衣艳君[1] 刘家尧[2] 骆爱玲[2] 张其德[2] 马德钦[3] 王学臣[4] 梁峥[2] 

机构地区：[1]曲阜师范大学生物系,曲阜273165 [2]中国科学院植物研究所,北京100093 [3]中国科学院微生物研究所,北京100081 [4]中国农业大学生物学院,北京100094

出　　处：《Acta Botanica Sinica》1999年第9期993-996,共4页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学重点基金;国家攀登计划;农业部重点项目;山东省自然科学基金

摘　　要：测定了导入甜菜碱醛脱氢酶（ＢＡＤＨ） 基因烟草（ Ｎｉｃｏｔｉａｎａｔａｂａｃｕｍ Ｌ．） 植株的叶绿素荧光诱导瞬变特性、呼吸酶和光呼吸酶的活性，并与亲本植株比较。结果表明，转基因植株的Ｆｖ／Ｆｏ 、Ｆｖ／Ｆｍ 和Ｆｄ／Ｆｓ 没有明显的变化；三羧酸循环中的苹果酸脱氢酶、异柠檬酸脱氢酶和琥珀酸脱氢酶活性略有增加；末端氧化的细胞色素氧化酶活性明显提高；光呼吸途径中的羟基丙酮酸还原酶、乙醇酸氧化酶和过氧化氢酶活性明显提高。对这些变化的可能意义进行了讨论。The transient characteristics of chlorophyll fluorescence induction, the activities of respiratory enzymes (malate dehydrogenase, isocitrate dehydrogenese, succinate dehydrogenase and cytochrome C oxidase) and the activities of photorespiratory enzymes (hydroxypyruvate reductase, glycolate oxidase and catalase) in the transgenic tobacco (Nicotiana tabacum L.), in which betaine aldehyde dehydrogenase (BADH) gene had been introduced, were determined and compared with the parent plants. The results showed that the F v/F o, F v/F m and F d/F s of the transgenic plant had no changes;the activities of malate dehydrogenase, isocitric dehydrogenase and succinic dehydrogenase in tricarboxylic acid cycle had a slight increase, and the activity of cytochrome C oxidase in the terminal oxidative pathway had a significant increase. The activities of hydroxypyruvate reductase, glycollic oxidase and catalase in the photorespiratory pathway had a marked increase. The possible significance on these changes was discussed.

关 键 词：甜菜碱醛脱氢酶 转基因烟草 呼吸酶 光系统 

分 类 号：Q785[生物学—分子生物学] S572.01[农业科学—烟草工业]