应用自由流电泳分离小鼠脾淋巴细胞  

Application of Free flow Electrophoresis to the Separation of Murine Lymphocytes

作　　者：刘韬[1] 沈欣 吴高德[1] 夏其昌[1] 王升年 吴直江[2] 胡伟 

机构地区：[1]中国科学院上海生物化学研究所,上海200031 [2]中国科学院上海细胞生物学研究所,上海200031

出　　处：《生物化学与生物物理学报》1999年第5期590-593,共4页

基　　金：国家载人航天工程项目

摘　　要：自由流电泳具有独特的全液相自由分离过程和非常温和的分离条件， 使用这种技术进行细胞等水不溶性活性物质的分离不仅效率高， 而且可以较好地保存其生物活性和成活力。应用自由流电泳在低离子强度的三乙醇胺（ Ｔ Ｅ Ａ） 缓冲液系统中进行了 Ｂ 和 Ｔ 淋巴细胞的分离， 并利用反向介质技术使分离后的细胞处于生理盐溶液中。通过紫外吸收光谱、免疫荧光标记和流式细胞仪对分离得到的各级分进行了检测， 结果证明 Ｂ 和 Ｔ 淋巴细胞在保持了较高成活力的同时得到了很好的分离。Free flow electrophoresis offers not only the all in solution separation process but also very gentle separation conditions, and it can be used for continuous separation and preparation, so it has been widely applied in the fields of biochemistry and cell biology. Using this technique to separate insoluble particles such as cells, both high separation efficiency and high activity and viability could be obtained. Murine lymphocytes were separated in low ionic strength triethanolamine buffer by free flow electrophoresis, the cell fractions were detected and characterized by means of UV spectrometry, immune fluorescence labeling and flow cytometry. Results indicated that T and B lymphocytes were well separated with high viability.

关 键 词：自由流电泳 分离 淋巴细胞 成活力 

分 类 号：Q2－33[生物学—细胞生物学]