First report of microcystin production in Microcystis smithii Komrek and Anagnostidis(Cyanobacteria) from a water bloom in Eastern China  

作　　者：Yang Liu Wenhua Tan Xingqiang Wu Zhongxing Wu Gongliang Yu Renhui Li 

机构地区：[1]Key Laboratory of Aquatic Biodiversity and Conservation Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China [2]Graduate University of Chinese Academy of Sciences, Beijing 100049, China

出　　处：《Journal of Environmental Sciences》2011年第1期102-107,共6页环境科学学报（英文版）

基　　金：supported by the State Key Basic Research and Development Plan of China (No.2008CB418002);the Frontier Research Project of the Chinese Academy of Science (No.082303-1-501)

摘　　要：A water bloom sample collected from Lake Dishui in Shanghai was characterized. The morphological identification showed that Micorcystis wesenbergii and Micorcystis smithii were the main component of the bloom. Five strains of M. smithii were successfully isolated. Their 16S rRNA gene sequences based phylogenetic tree showed that the five strains of M. smithii intermixed with strains of other morphospecies in Microcystis. A fragment of mcy gene encoding for microcystin synthetase was detected in one of the five M. smithii strains （CHAB 2183）, indicating its potential of microcystin production. High performance liquid chromatography analysis confirmed M. smithii CHAB 2183 to produce microcystin-RR as 1550 μg per gram dry weight cells. The present investigation, for the first time, reported the isolated strains of M. smithii and microcystin production from M. smithii.A water bloom sample collected from Lake Dishui in Shanghai was characterized. The morphological identification showed that Micorcystis wesenbergii and Micorcystis smithii were the main component of the bloom. Five strains of M. smithii were successfully isolated. Their 16S rRNA gene sequences based phylogenetic tree showed that the five strains of M. smithii intermixed with strains of other morphospecies in Microcystis. A fragment of mcy gene encoding for microcystin synthetase was detected in one of the five M. smithii strains （CHAB 2183）, indicating its potential of microcystin production. High performance liquid chromatography analysis confirmed M. smithii CHAB 2183 to produce microcystin-RR as 1550 μg per gram dry weight cells. The present investigation, for the first time, reported the isolated strains of M. smithii and microcystin production from M. smithii.

关 键 词：CYANOBACTERIA Microcystis smithii MICROCYSTIN-RR mcyA 16S rRNA gene 

分 类 号：Q959.4[生物学—动物学] X52[环境科学与工程—环境工程]