人外周血和扁桃体中Tfh细胞与其他Th细胞亚群之间的关系  

Relationship between Tfh cells and the other Th cell subsets in human peripheral blood and tonsils

作　　者：冯敛[1] 刘昀[1] 王慧[1] 李祖望[2] 李华斌[2] 吴长有[1] 

机构地区：[1]中山大学医学院免疫学教研室热带传染病教育部重点实验室,广州510080 [2]中山大学第一附属医院耳鼻喉医院

出　　处：《免疫学杂志》2011年第1期42-48,共7页Immunological Journal

基　　金：国家重点基础研究发展计划(973)资助项目(2007CB512404)

摘　　要：目的比较观察人外周血和扁桃体Tfh细胞的表型以及与Th1、Th17、Th22细胞亚群之间的关系。方法分离正常人PBMC及扁桃体单个核细胞,利用anti-CD3+anti-CD28或PMA+ionomycin刺激后,采用ELISA和流式细胞术(FCM)检测其细胞因子的产生,分析Tfh与Th1、Th17、Th22细胞亚群之间的关系。结果与PBMC中CD4+T细胞不同,扁桃体CD4+T细胞高表达CXCR5和CD45RO,低表达CCR7和CD62L;与PBMC中CD4+T细胞相比,扁桃体CD4+T细胞IL-21和IL-17产生水平较高,IFN-γ产生水平较低,IL-22水平无显著差异;外周血和扁桃体CD4+T细胞中均存在一定比例的IL-21+IL-17+双阳性、IL-21+IL-22+双阳性、IL-21+IFN-γ+双阳性细胞,IL-21单阳性细胞在扁桃体CD4+T细胞中所占比例明显高于外周血;外周血和扁桃体CD4+CXCR5+细胞除表达IL-21外,还表达IL-17、IL-22和IFN-γ。结论扁桃体中存在较多数量的Tfh细胞,大多数Tfh细胞是不同于Th1、Th17和Th22的细胞亚群。This paper aimed to compare the phenotypes of Tfh cells as well as evaluate the relationship of Tfh cells with Th1,Th17 and Th22 cell subsets in human peripheral blood and tonsils.We isolated PBMCs and tonsillar mononuclear cells,then detected the production of cytokines by ELISA and FACS after stimulation with anti-CD3 plus anti-CD28 or PMA plus ionomycin.Finally,the relationship of Tfh cells with Th1,Th17 and Th22 cell subsets was identified.Different from what observed in PBMCS,higher expression of CXCR5 and CD45RO and lower expression of CCR7 and CD62L were demonstrated in tonsillar CD4＋ T cells.Tonsillar CD4＋ T cells produced higher levels of IL-21 and IL-17 but lower level of IFN-γ compared with CD4＋ T cells in PBMCs.While the level of IL-22 was similar in PBMC and tonsillar CD4＋ T cells.A few of IL-21＋ IL-17＋,IL-21＋ IL-22＋ and IL-21＋ IFN-γ＋ cells were existed both in PBMC and tonsillar CD4＋ T cells.Furthermore,the frequencies of IL-21 single positive cells were higher in tonsillar CD4＋ T cells than in CD4＋ T cells from PBMCs.Besides co-expression with IL-21,some proportion of CD4＋ CXCR5＋ cells in PBMCs and tonsils are IL-17-positve,IL-22-positive or IFN-γ-positive.We concluded that there are higher amount of Tfh cells in tonsils than in PBMCs,and the majority of Tfh cells belong to a distinct subset from Th1,Th17,or Th22 cells.

关 键 词：滤泡辅助性T细胞 CXCR5 IL-21 TH细胞亚群 

分 类 号：R392.1[医药卫生—免疫学]