rSj26-Sj32融合蛋白用于急性日本血吸虫病患者的诊断价值  

Diagnostic value of rSj26-Sj32 in patients with acute schistosomiasis japonicum

作　　者：王敏[1] 李文桂[1] 蔡世飞[1] 

机构地区：[1]重庆医科大学附属第一医院传染病寄生虫病研究所,400016

出　　处：《免疫学杂志》2011年第1期49-53,69,共6页Immunological Journal

基　　金：重庆市科委地方病重大专项基金(2008AB5055;2008AB5008;2008AB5054)

摘　　要：目的探讨rSj26-Sj32融合蛋白用于急性日本血吸虫病患者的诊断价值。方法以纯化获得的rSj26-Sj32融合蛋白和SjAWA为包被抗原建立酶联免疫吸附试验(ELISA)、斑点酶联免疫吸附试验(Dot-ELISA)和Dipstick三种方法分别检测急性日本血吸虫病患者血清中IgG抗体,并以华支睾吸虫病、卫氏并殖吸虫病、泡型棘球蚴病、囊型棘球蚴病、乙型肝炎和肺结核患者以及健康人血清作对照。结果 rSj26-Sj32三种方法检测急性日本血吸虫病患者血清的敏感性分别为90.00%、100.00%和96.00%;特异性分别为97.67%、95.35%和97.67%。结论 rSj26-Sj32融合蛋白作为诊断抗原用于急性日本血吸虫病患者血清的诊断具有较好的敏感性和特异性,对急性日本血吸虫病的诊断有较大的应用价值。We aimed to investigate the diagnostic value of rSj26-Sj32 in acute schistosomiasis japonicum.ELISA,Dot-ELISA and Dipstick were built by the purified rSj26-Sj32 protein and Schistosoma japonicum adult worm antigen（SjAWA） to detected the IgG levels in sera of patients acute schistosomiasis japonicum,while controls were set with the patients with clonorchiasis sinensis,paragonimiasis westermani,alveolar echinococcosis,cystic echinococcosis,hepatitis B,or lung tuberculosis as well as the health donors.As the results,the sensitivity of rSj26-Sj32-ELISA,rSj26-Sj32-Dot-ELISA and rSj26-Sj32-Dipstick were 90.00%,100.00% and 96.00% in diagnosing acute schistosomiasis japonicum,respectively,while the specificity were 97.67%,95.35% and 97.67% respectively.The rSj26-Sj32 antigen shows high sensitivity and specificity for the diagnosis of acute schistosomiasis japonicum,which might be used as a diagnostic antigen for acute schistosomiasis japonicum.

关 键 词：日本血吸虫病 rSj26-Sj32融合蛋白 免疫诊断 

分 类 号：R532.21[医药卫生—内科学]