microRNA-16 expression in HeLa cells is upregulated in cell-cycle and drug dependent manner  

作　　者：LI Jie WU JiaRui 

机构地区：[1]Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science & Technology of China, Hefei 230027, China [2]Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China

出　　处：《Chinese Science Bulletin》2011年第1期34-38,共5页

基　　金：supported by the National Key Basic Research and Development Program of China (2006CB503900);National Natural Science Foundation of China (30821065);Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX1-YW-02 and KJCX2-YW-M15); Science and Technology Commission of Shanghai Municipality (07dz05907)

摘　　要：microRNAs are single-stranded,non-coding RNAs that regulate gene expression.The microRNA-16 family has been reported to be involved in cell-cycle regulation,which could also downregulate expression of multiple pro-proliferation genes.The present results demonstrated that miR-16 expression in HeLa cells increased when the cells were arrested during S-phase under methyl methanesulfate (MMS) treatment.This further resulted in downregulation of a target protein CDC25A,whereas miR-16 expression did not increase when HeLa cells were arrested during the MMS-treated G0/G1 or G2/M phase.Furthermore,when HeLa cells were arrested during S-phase with hydroxyurea treatment,miR-16 expression did not increase.These results suggest that expression levels of microRNAs in mammalian cells are delicately regulated under variable cellular conditions.microRNAs are single-stranded, non-coding RNAs that regulate gene expression. The microRNA-16 family has been reported to be involved in cell-cycle regulation, which could also downregulate expression of multiple pro-proliferation genes. The present results demonstrated that miR-16 expression in HeLa cells increased when the cells were arrested during S-phase under methyl methanesulfate （MMS） treatment. This further resulted in downregulation of a target protein CDC25A, whereas miR-16 expres- sion did not increase when HeLa ceils were arrested during the MMS-treated G0/G1 or G2/M phase. Furthermore, when HeLa cells were arrested during S-phase with hydroxyurea treatment, miR-16 expression did not increase. These results suggest that expres- sion levels of microRNAs in mammalian cells are delicately regulated under variable cellular conditions.

关 键 词：HELA细胞 细胞周期调控 小分子RNA microRNA 依赖性 药物 非编码RNA 哺乳动物细胞 

分 类 号：Q255[生物学—细胞生物学] Q253