Sebia全自动毛细管电泳仪的临床应用评价  

The clinical applications of Sebia automatic capillary electrophoresis

作　　者：刘素兰[1] 唐中[1] 蒋兴亮[1] 张均[1] 胡帅[1] 

机构地区：[1]川北医学院附属医院检验科,四川南充637000

出　　处：《医学信息（医药版）》2011年第1期27-29,共3页

摘　　要：目的：分析Sebia全自动毛细管电泳仪在几种疾病中的血清蛋白电泳结果，以探讨其临床应用价值。方法：对肝脏疾病组（肝硬化、肝癌）55例；肾脏疾病组（肾病综合征、慢性肾炎）60例1；自身免疫性疾病组（类风湿性关节炎、多发性骨髓瘤）27例；急性炎症组（阑尾炎、胰腺炎、慢阻肺）50例及对照组100例（来自本院健康体检者）。均由早晨空腹采集标本，分离血清后进行Sebia全自动毛细管电泳，并对电泳结果进行比较。同时进行批内、批间重复性试验、线性试验及干扰试验。结果：除肾脏疾病组各项结果与对照组有明显差异（P〈0．001）外，其余肝脏疾病组、自身免疫性疾病组、急性炎症组与对照组比较部分项目有明显差异（P〈0．001）。批内、批间重复性试验显示，与说明书一致。线性试验显示，白蛋白在4．8g／L，1在0．63g／L时均能电泳出结果。干扰试验显示，标本溶血，尤其严重溶血时，对电泳结果影响大，脂血、黄疸标本对结果基本无影响。结论：通过对肝脏疾病、肾脏疾病、自身免疫性疾病及急性炎症等患者血清蛋白电泳，同时进行批内、批间重复性试验、线性试验、干扰试验，认为Sebia全自动毛细管电泳仪为临床提供了快速、灵敏、可靠、准确的诊断依据。Objective The Sebia automated multicapillary electrophoresis was used for serum protein analysis of several diseases. Disscuss its＇ practical application in clinical. Methods The Sebia automated multicapiUary electrophoresis was used for serum protein analysis of liver disease Group （cirrhosis and hepatocellular carcinoma） 55 cases; kidney disease groups （nephrotic syndrome, chronic nephritis） 60 cases; autoimmune disease Group （rheumatoid arthritis, multiple myeloma） 27 cases; acute inflammation Group （appendicitis, pancreatitis, chronic obstructive pulmonary disease） 50 cases and normal group （health checkup）100 cases, and compared with the results. Batch and between batches repeatability test. linearity and Interference were analyzed. Results There were significant differences （P 〈0.001） in the various results except for kidney disease, within-run or between-run show similar as brochures, linearity show, albumin in 4.8g/L, y in 0.63g/1 are able to electrophoretic analysis. Interference test displays, there was affect with hemolysis, particularly acute hemolysis, there was no affect with lipidemia and jaundice. Conclusion The Sebia automated multicapillary electrophoresis was used for serum protein analysis to provide rapid, responsive, reliable, accurate diagnosis for clinical.

关 键 词：Sebia 全自动毛细管电泳仪:临床应用 

分 类 号：R446.112[医药卫生—诊断学]