野生和养殖鲻消化酶活性的比较研究  

Digestive enzymes activity of wild and cultivated grey mullet Mugil cephalus

作　　者：于娜[1,2] 李加儿[1] 区又君[1] 范春燕[1,2] 

机构地区：[1]中国水产科学研究院南海水产研究所,广东广州510300 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《中国水产科学》2011年第1期127-135,共9页Journal of Fishery Sciences of China

基　　金：广东省科技计划项目(2007B020708005)

摘　　要：对野生鲻(Mugil cephalus)和养殖鲻的消化道指数及淀粉酶和蛋白酶活性分布进行了比较研究,并探讨了不同温度和pH对离体状态下消化道淀粉酶和蛋白酶的影响。结果表明,野生鲻和养殖鲻的比内脏重、比肝重、比胃重、比肠长分别为0.043±0.001和0.0480±0.004,0.009±0.001和0.007±0.000,0.008±0.002和0.007±0.000,2.886±0.301和3.158±0.141。野生鲻的蛋白酶和淀粉酶活力在各消化器官中的分布与养殖鲻间存在差异。野生鲻和养殖鲻比较,野生鲻各消化器官中淀粉酶活力均极显著高于养殖鲻(P<0.01);蛋白酶活力在肝胰脏和胃中差异不显著(P>0.05),在中肠差异显著(P<0.05),在前肠和后肠差异极显著(P<0.01)。在肝胰脏、胃、前肠、中肠、后肠和幽门盲囊6部位,野生鲻淀粉酶的最适温度分别是40℃、45℃、45℃、45℃、40℃、45℃;养殖鲻淀粉酶的最适温度分别是40℃、40℃、40℃、45℃、40℃、40℃。野生鲻淀粉酶最适pH在肝胰脏为6.2,在其余各部分均是7.2;养殖鲻淀粉酶最适pH在胃和前肠为2.2和8.0,在其余各部分均为7.2。野生鲻蛋白酶最适温度在肝胰脏、胃、前肠和后肠均为45℃,在中肠为50℃;养殖鲻蛋白酶最适温度在肝胰脏、胃和中肠为40℃,前肠和后肠为45℃。野生和养殖鲻蛋白酶的最适pH胃部为3.2,其余各部分的最适pH均是7.2。Digestive tract indices and digestive enzyme distribution in digestive organs of wild and cultivated grey mullet Mugil cephalus were studied,and effects of different temperature and pH on digestive enzyme activities were investigated in both populations.The results showed that in wild and cultivated grey mullets,the ratio of intestine gut length to body length were 2.886±0.301 and 3.315±0.141 while the ratios of viscera,liver and stom-ach weight to body weight were 0.043±0.001 and 0.048±0.004,0.009±0.001 and 0.007±0.000,0.008±0.002 and 0.007±0.000,respectively.The activities of protease in different organs were sequenced as follow： hindgut〉foregut〉midgutst〉omach〉hepatopancreas in wild grey mullets and foregut〉midgut〉hindgut〉 hepato-pan-creasstomach in cultured ones,while the activities of amylase were in the order of hindgut pyloric caecum〉midgut〉foregut〉hepatopancreas〉stomach in wild grey mullets and pyloric caecum foregutmidgut hindgut〉stomach 〉hepatopancreas in cultured ones.The differences of amylase activity in every part between the populations were extremely significant（P〈0.01）.No significant differences of protease activity in hepatopancreas and stomach exited between the two populations（P〉0.05）,but in midgut the difference was significant（P〈0.05）,and in foregut and hindgut,the difference were extremely significant（P〈0.01）.For the amylase in wild grey mullet,the optimum temperatures were 40℃,45℃,45℃,45℃,40℃,45℃ in hepatopancreas,stomach,foregut,midgut,hindgut and pyloric caecum,respectively and the optimum pH in hepatopancreas was 6.2 and in other tissues was 7.2.For amylase in cultured grey mullet,the optimum temperatures were 40℃,40℃,40℃,45℃,40℃,40℃ in hepatopancreas,stomach,foregut,midgut,hindgut and pyloric caecum,respectively and the optimum pH in stomatch and foregut were 2.2 and 8.0,and in other tissues was 7.2.For protease in wild grey mullet,the optimum temperature in hepatopancreas,stomach,foregut and hindgut was

关 键 词：鲻 消化道指数 消化酶分布 酶活 温度 PH 

分 类 号：S917[农业科学—水产科学]