HpaA-VacA IgY对小鼠胃内感染幽门螺杆菌的预防作用  

Preventive Effect of HpaA-VacA IgY on Intragastric Infection with Helicobacter pylori in Mice

作　　者：夏丽君[1] 杨致邦[2] 黄伟[3] 张任飞[1] 蒋仁举[1] 蒋英[1] 

机构地区：[1]重庆医科大学基础医学院神经科学研究中心,重庆400016 [2]重庆医科大学基础医学实验教学中心病原生物学与免疫学实验室,重庆400016 [3]西南大学荣昌校区动物医学系,重庆402460

出　　处：《中国生物制品学杂志》2011年第1期34-36,40,共4页Chinese Journal of Biologicals

基　　金：重庆市科委基金资助项目(CSTC;2005EA5020)

摘　　要：目的 研究HpaA-VacA IgY对小鼠幽门螺杆菌(Helicobacter pylori,H.pylori)胃内定植及引起炎症的预防作用,为制备集预防和治疗H.pylori感染为一体的IgY制剂奠定基础。方法将BALB/c小鼠随机分为阳性对照组、阴性对照组、感染预防组和炎症预防组,阳性对照组灌喂含108 cfu/ml H.pylori的菌液;阴性对照组灌喂布氏肉汤代替H.pylori菌液;感染预防组灌喂不同剂量的IgY后,再灌喂同等剂量的菌液;炎症预防组灌喂同等剂量的菌液后,再灌喂不同剂量的IgY。通过胃黏膜H.pylori培养和组织切片染色镜检观察H.pylori定植及炎症反应程度。结果 HpaA-VacA IgY剂量为6 mg/只时,对H.pylori感染的预防率为88.9%,预防效果较好,当HpaA-VacA IgY的剂量为8 mg/只时,能完全预防H.pylori感染及其所引起的胃黏膜炎症。结论小鼠口服HpaA-VacA IgY具有预防和治疗H.pylori感染的作用,可用于制备防治H.pylori感染的口服制剂。Objective To study the preventive effect of HpaA-VacA IgY on the intragastric colonization of Helicobacter pylori and the subsequent inflammation in mice,and lay a foundation of developing IgY-related drugs for both prevention and therapy of H. pylori infection. Methods BALB / c mice were randomly divided into positive control,negative control,infection-preventing and inflammation-preventing groups. The mice in positive control group were administered with 108 cfu / ml H. pylori liquid by intragastric infusion,while those in negative control group with Brucella broth. The mice in infection-preventing group were administered with IgY at various dosages,then with 108 cfu / ml H. pylori liquid. However,the mice in inflammation-preventing group were administered with 108 cfu / ml H. pylori liquid,then with IgY at various dosages. The colonization of H. pylori and severity of inflammatory reaction were observed by culture of intragastric H. pylori and microscopy of stained histological sections. Results The preventionrate of H. pylori infection by HpaA-VacA IgY at a dosage of 6 mg was 88. 9%. However,the HpaA-VacA IgY at a dosage of 8 mgcompletely protected the mice against H. pylori infection and the subsequent inflammation. Conclusion Oral administration with HapA-VacA IgY showed both preventive and curative effects on H. pylori infection in mice,indicating that the IgY might be used for preparation of oral drug for H. pylori infection.

关 键 词：幽门螺杆菌 细胞空泡毒素 黏附素 卵黄抗体 体内试验 

分 类 号：R378[医药卫生—病原生物学] R392-33[医药卫生—基础医学]