LHRH-PE40对黑色素瘤A375细胞的诱导凋亡作用  

Luteinizing Hormone Releasing Hormone-Pseudomonas aerugionosa Exotoxin 40 Induces Apoptosis of Melanocytoma A375 Cells

作　　者：邓欣[1] 刘莹[2] 张国利[3] 朱平[3] 宋阳[1] 

机构地区：[1]中国医科大学基础医学院机能实验中心,沈阳110001 [2]中国医科大学基础医学院生物化学与分子生物学教研室,沈阳110001 [3]军事医学科学院军事兽医研究所生物技术应用研究室,长春130062

出　　处：《中国生物制品学杂志》2011年第1期37-40,共4页Chinese Journal of Biologicals

基　　金：国家科技部科技型中小企业创新基金(03C26212200869);辽宁省教育厅高校科研项目计划(2008738)

摘　　要：目的 研究促黄体激素释放激素-绿脓杆菌外毒素(Luteinizing hormone releasing hormone-Pseudomonas aeru-gionosa exotoxin 40,LHRH-PE40)对黑色素瘤A375细胞的诱导凋亡作用。方法用LHRH-PE40处理A375细胞,透射电镜观察细胞结构的改变;DNA ladder和流式细胞仪检测细胞的凋亡。结果经LHRH-PE40作用后,透射电镜观察可见A375细胞核聚集于一侧;DNA ladder检测发现,A375细胞的DNA电泳图像呈梯形条带;流式细胞术检测结果显示,A375细胞出现明显的二倍体峰,细胞周期也发生了相应的改变。结论 LHRH-PE40对A375细胞的细胞毒性作用可以有效诱导A375细胞凋亡。Objective To investigate the role of luteinizing hormone releasing hormone-Pseudomonas aerugionosa exotoxin 40 （LHRH-PE40）in induction of apoptosis of melanocytoma A375 cells. Methods A375 cells were treated with LHRH-PE40,then observed for structural change by transmission electron microscopy and determined for apoptosis by DNA ladder and flow cytometry.Results Under transmission electron microscope,the nuclei of A375 cells after treatment with LHRH-PE40 were aggregated to the margin of nuclear membrane. DNA ladders were observed on the DNA electrophoretic profile of A375 cells treated with LHRH-PE40.Flow cytometry showed obvious sub-diploid peaks of A375 cells,while the cell cycle changed accordingly. Conclusion The cytotox-icity of LHRH-PE40 to A375 cells induced the cell apoptosis effectively.

关 键 词：促黄体激素释放激素-绿脓杆菌外毒素(LHRH-PE40) 黑色素瘤 毒性作用 细胞凋亡 促黄体激素释放激素受体(LHRHR) 

分 类 号：R966[医药卫生—药理学]