雷公藤内酯醇对浆细胞样树突状细胞功能及成熟的影响  

Regulation on function and maturation of plasmacytoid dendritic cells by triptolide

作　　者：刘立民[1] 焦雯静 张兴霞[1] 张彦明[1] 赵广圣[1] 崔红霞[1] 孙雨梅[1] 陆沭华[1] 

机构地区：[1]淮安市第二人民医院血液科徐州医学院附属淮安医院,223002 [2]陕西省咸阳市中心医院血液科

出　　处：《中华微生物学和免疫学杂志》2011年第1期6-9,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的 通过体外试验研究雷公藤内酯醇（triptolide）对浆细胞样树突状细胞（plasmacytoid dendritic cell,pDC）功能及成熟的影响,为进一步阐明雷公藤内酯醇的免疫学活性提供依据.方法 从健康志愿者外周血分离单个核细胞,流式细胞仪分选pDC,加入0、5、10、30 μg/L的雷公藤内酯醇共孵育,24 h后收集上清液,ELISA检测pDC分泌的IFN-α、IL-6、TNF-α量,5 d后收集细胞,流式细胞仪检测树突状细胞表型CD11c、CD80、CD86阳性率,光镜观察DC的形态,扫描电镜观察DC的超微结构.结果 雷公藤内酯醇显著降低pDC分泌的IFN-α、IL-6、TNF-α,并呈雷公藤内酯醇浓度依赖性（P＜0.05）;雷公藤内酯醇可抑制pDC向DC的分化和成熟,并呈雷公藤内酯醇浓度依赖性（P＜0.05）.结论 雷公藤内酯醇能够降低pDC的功能,并抑制其向DC的分化和成熟.Objective To explore the mechanism of immunomodulatory activity of triptolide on healthy volunteers peripheral blood mononuclear cells （PBMC）-derived plasmacytoid dendritic cells （pDCs）. Methods Healthy volunteers-derived pDCs were sorted by flow cytometry, then incubated with triptolide （0, 5, 10, 30 μg/L）. After 24 hours, we detected the concentration of IFN-α, IL-6, TNF-α using ELISA. After 5 days, the cultrural cells were collected and analyzed by flow cytometry, light microscope and electron microscope scanning. Results Triptolide-treated pDCs secreted lower level of IFN-α,IL-6 ,TNF-α, triptolide could inhibit pDCs differentiation to DCs which displayed more immature morphology and immunophenotypes than untreated-pDCs. Conclusion Triptolide could decrease the immune function of pDCs, inhibit differentiation and maturation of pDCs.

关 键 词：雷公藤内酯醇 浆细胞样树突状细胞 成熟 

分 类 号：R285[医药卫生—中药学]