肺炎克雷伯菌的喹诺酮外排基因qepA的检测及菌株同源性分析  

Study of qepA among clinical isolates of Klebsialla pneumoniae

作　　者：马晓波[1] 林粼[2] 宋秀宇[1] 房丽丽[1] 张加勤[1] 郑港森[1] 郑燕青[1] 

机构地区：[1]厦门大学附属第一医院检验科,361003 [2]厦门大学附属第一医院中心实验室,361003

出　　处：《中华微生物学和免疫学杂志》2011年第1期47-50,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的 检测质粒介导的喹诺酮类外排基因qepA在临床分离的产超广谱β-内酰胺酶（ESBLs）的肺炎克雷伯菌中的分布情况,并对阳性菌株进行同源性分析.方法 41株细菌的鉴定和药敏采用Vitek-2 Compact系统;采用PCR法检测qepA并进行序列测定、比对.应用Rep-PCR法采用Diversilab系统对qepA阳性菌株进行同源性分析;同时了解阳性株上的rmtB分布情况.结果 5株肺炎克雷伯菌上检测到qepA基因,检出率为12.2%.Diversilab分析结果表明其中两株的qepA阳性菌同源性超过99%.包含qepA的肺炎克雷伯菌中2株（40%）检出rmtB.结论 在肺炎克雷伯菌临床菌株上检出质粒介导的喹诺酮类外排基因qepA.Objective To investigate the prevalence of qepA, quinolone efflux protein, among 41 unique clinical strains of K. pneumoniae producing ESBLs and to study the qepA-bearing isolates using the Diversilab system. Methods Identification and antimicrobial susceptibility were performed by Vitek-2 Compact System. Screening of qepA was carried out by PCR amplification. The NCBI BLAST program was utilized for sequence comparisons. qnr-bearing strains was evaluated by the Repetitive-sequence-based PCR（Rep-PCR） employing the Diversilab system. And the existence of rmtB was detected among these qepA contained isolates. Results qepA were detected in 5 isolates（ 12.2% ）. The Rep-PCR profiles produced by the Diversilab system showed that 2/5 of isolates were indistinguishable. And 60% of qepA-positive isolates were detected to harbor rmtB gene. Conclusion The data suggest the emergence of qepA-borne K. pneumoniae.

关 键 词：质粒介导 QEPA 肺炎克雷伯菌 

分 类 号：R440[医药卫生—诊断学]