输注供者抗原特异性调节性T淋巴细胞延长小鼠胰岛移植物的存活时间  

Infusion of donor-specific regulatory T cells prolonged islet allograft survival in mice

作　　者：李明[1] 陈栋[1] 李永海[1] 昌盛[1] 向莹[1] 郭晖[1] 张伟杰[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院器官移植研究所,武汉430030

出　　处：《中华器官移植杂志》2011年第1期20-23,共4页Chinese Journal of Organ Transplantation

摘　　要：目的 研究体外扩增获得的供者抗原特异性调节性T淋巴细胞（Treg细胞）对小鼠同种胰岛移植物存活时间的影响.方法 建立从Balb/c小鼠到C57小鼠的胰岛移植模型,通过观察移植后小鼠血糖情况来判断移植胰岛的存活时间.受鼠制成糖尿病模型后分为3组.对照组：不给予任何处理,只观察血糖变化;单纯胰岛移植组：移植胰岛,但不输注Treg细胞;实验组：术前1 d静脉给予1×106个供者特异性Treg细胞,然后行胰岛移植.结果胰岛移植后,对照组小鼠血糖持续高于16.7 mmol/L;单纯胰岛移植组小鼠血糖于术后1～2 d全部降至正常,到7～11 d时陆续开始升高,并维持在术前水平,存活时间为（8.50±1.6）d;实验组小鼠血糖于术后2 d内降至正常,之后维持在较低水平,至第21天开始有小鼠血糖升高超过16.7 mmol/L,存活时间为（26.2±3.9）d,明显长于单纯胰岛移植组（P＜0.05）.结论 输注供者抗原特异性Treg细胞可以明显延长移植胰岛的存活时间,在胰岛移植耐受中有积极的作用.Objective To investigate the effects of donor-specific regulatory T cells （Treg） transfusion on islet allograft survival. Methods Allogeneic fresh islets from Balb/c mice were transplanted to streptozotocin-induced diabetic C57 mice. The survival of islet allografts was observed. The experiment was divided into 3 groups： control group, nothing had been done to the recipients; simple islet transplantation group, the recipients received the islet transplantation only; experimental group, the recipients were given 1 ×106 Treg, then received islet transplantation. Results Blood glucose （BG） was above 16. 7 mmol/L after islet transplantation in control group; In simple islet transplantation group,BG level returned to normal level 1 to 2 days after transplantation, and hyperglycemia appeared 7 to 11 days after transplantation and maintained as the same as that before transplantation; In experimental group, BG level returned to normal level 2 days after transplantation and maintained at a low level,and at the 21st day after transplantation BG level was over 16. 7mmol/L in some recipients. Islet allograft survival in experimental group was significantly prolonged as compared with simple islet transplantation group. Conclusion Donor-specific Treg transfusion could prolong the islet allograft survival,and maybe have positive effect on tolerance induction of islet transplantation.

关 键 词：小鼠 胰岛移植 调节性T淋巴细胞 糖尿病 

分 类 号：R657.5[医药卫生—外科学]