广东屠猪肉样品中大肠杆菌耐药性与毒力特征的分析  

Antimicrobial resistance and virulence factors of Escherichia coil isolated from pork in Guangdong provoince

作　　者：孙迎[1] 廖晓萍[1] 王秀梅[2] 孙坚[1] 刘宝涛[1] 朱恒乾[1] 张悦[1] 王杨[1] 张美君[1] 刘雅红[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/细菌病研究室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2011年第1期32-36,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金"广东省政府自然科学基金联合基金重点项目"(U0631006);广东省自然科学基金创新团队项目(5200638);教育部创新团队项目(IRT0723)

摘　　要：为分析广东地区屠猪肉中大肠杆菌(E.coli)药物敏感菌株的血清型、毒力基因和系统进化背景,本研究从屠猪肉样品中分离出112株E.coli,采用玻片凝集法鉴定血清型,琼脂稀释法测定10种抗菌药的敏感性,PCR方法检测7种毒力相关基因,多重PCR方法进行系统进化背景判定。结果显示,112株E.coli中,定型菌株95株,分别属于15种血清型,其中O65、O131、O8和O158为优势血清型。几乎所有菌株对氟苯尼考、多西环素和四环素高度耐药,而对头孢曲松高度敏感,其中多重耐药菌株多数耐5种以上药物,常见的多重耐药表型是氟苯尼考/氯霉素/多西环素/四环素/氨苄西林。PCR鉴定结果表明,含有毒力基因的菌株中38%至少具有两个毒力基因,其中EAST1+Stx2e和hlyF+Stx2e比较常见。比较常见的毒力基因为Stx2e和EAST1,STb基因仅在一株菌中检测到。多重PCR鉴定结果显示,屠猪肉样品中E.coli主要分布为共生型的A组和B1组。本研究为大肠杆菌病的控制和合理使用抗生素提供实验依据。This study investigated the prevalence of antimicrobial resistance serotypes, virulence genes and phylogenetic background of 112 Escherichia coil isolates from pork in Guangdong area. The O serotypes were identified by slide agglutination tests, the resistance phenotypes were tested by agar dilution. All isolates were screened for virulence genes by PCR, and the phylogenetic background determinated by multiplex PCR. Fifteen O serotypes were identified from 95 E. coli isolates, of which 065, O131, 08 and O158 were dominant serogroups. Almost all isolates were highly resistant to florfenicol, doxycycline, and tetracycline, but susceptible to ceftriaxone. Multiple antimicrobial-resistant phenotypes （≥ 5 antimicrobials） were found in 79 isolates of E. coil strains. The most detectied virulence genes were stx2e and EAST1, with 38% positive isolates carded two or more virulence genes. The combinations of EASTI＋Stx2e and hlyF＋Stx2e were more than others. The majority of isolates belonged to phylogenetic group A and B1.

关 键 词：大肠杆菌 耐药性 血清型 毒力基因 

分 类 号：S852.61[农业科学—基础兽医学]