猪瘟病毒E2抗原在不同酶标板上包被效果的比较  

Comparison of coating capacity of CSFV E2 on different polystyrene plates

作　　者：宋艳[1,2] 单晶辉[1] 张茂林[1,3] 段铭[1] 关振宏[1] 李宁[1] 卢强[1] 涂长春[4] 

机构地区：[1]吉林大学人兽共患病研究所人兽共患病研究教育部重点实验室,吉林长春130062 [2]北华大学护理学院,吉林吉林132011 [3]广西民族大学化学与生态工程学院,广西南宁530006 [4]军事医学科学院军事兽医研究所,吉林长春130062

出　　处：《中国预防兽医学报》2011年第1期73-75,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：吉林省科技发展计划重点项目(20080930);广西科学基金[桂科(自)0728057];吉林大学农学部引进人才科研启动基金(4305050102C1)

摘　　要：为比较杆状病毒表达的猪瘟病毒(CSFV)E2抗原在不同酶标板上的包被效果,本研究应用2μg/mL的CSFV E2分别包被Nunc Maxisorp、Cosar StripWell和JET FEP-101-896 3种酶标板,以Flyer Liquid Plate Sealer(FLPS)进行封闭,通过比较猪瘟阳性血清的OD450nm值,筛选包被性能最佳的酶标板。对FLPS、含10%FCS的PBST和含1%BSA的PBST的封闭效果进行比较。并比较FLPS在相同抗原条件下重复使用2次和3次对于CSFV阳性血清检测结果的影响。结果显示,在CSFV E2抗原包被的3种酶标板上,CSFV阳性血清的OD450nm值均存在显著差异(p<0.05),其中以Cosar酶标板OD450nm值最高;在Costar板上,3种封闭液间及FLPS重复使用对于CSFV阳性血清的检测结果无显著差异(p>0.05)。In order to evaluate the affinity property of ELISA plate to baculoviruses-expressed glycoprotein E2 of classical swine fever virus, three commercial ELISA plates, Nunc Maxisorp, Cosar StripWell and JET plates were selected and coated with CSFV E2 antigen at 2 txg/mL. After flyer liquid plate sealer （FLPS） blocking, the highly sensitive plate was screened by CSFV positive pig sera, followed by treatment with other blocking agents, such as PBST （10% FCS） and PBST （1% BSA）. The second and third use of FLPS were also evaluated on the selected plates, respectively. The results showed that there were significant differences among the plates （p〈0.05）, and the most sensitive data was obtained on costar plate, and no significant variations were founded among the used blocking agents （p〉0.05）.

关 键 词：猪瘟病毒E2抗原 酶标板 包被效果 

分 类 号：S852.5[农业科学—基础兽医学]