低氧诱导因子1α在继发性脊髓损伤中的时序性表达及意义  

SEQUENTIAL EXPRESSION OF HYPOXIA-INDUCIBLE FACTOR 1α AND ITS SIGNIFICANCE IN SECONDARY SPINAL CORD INJURY

作　　者：牛庆飞[1] 贾长青[2] 王楠[1] 陈晓春[1] 赤仁杰[1] 柏树令[3] 

机构地区：[1]沈阳医学院奉天医院骨外科,沈阳110024 [2]中国医科大学附属盛京医院脊柱关节外科 [3]中国医科大学组织工程教研室

出　　处：《中国修复重建外科杂志》2011年第1期91-95,共5页Chinese Journal of Reparative and Reconstructive Surgery

摘　　要：目的通过观察大鼠继发性脊髓损伤(spinal cord injury,SCI)中低氧诱导因子1α(hypoxia-inducible factor lα,HIF-lα)的表达规律,探讨其在继发性SCI发生、发展过程中的作用。方法 SD大鼠66只,雌雄不限,体重(250±20)g,随机分为正常对照组(A组,6只)、假损伤组(B组,6只)和SCI组(C组,54只)。A组不作任何处理;B组大鼠仅行椎板切除术;C组大鼠在椎板切除术后采用脊髓静压法建立T10静压SCI模型。A、B组于术后1h,C组于术后1、3、6、12h及1、2、3、7、14d各处死6只大鼠,取损伤区脊髓组织行HE染色,采用SABC法行免疫组织化学染色观察各组HIF-1α阳性表达情况并计数。结果各组动物均存活至实验完成。HE染色示,A、B组脊髓组织结构致密,细胞核圆而大、染色浅、核仁清晰。C组术后1～12h,损伤局部神经元核固缩,胞体缩小,胞质深伊红染色;1～3d,轴突肿胀,出现空泡,白质内神经髓鞘散乱、崩解破裂;7、14d,胶质细胞增生明显,损伤段脊髓变细,部分灰质崩解坏死,可见囊腔形成。免疫组织化学染色示,A组HIF-1α阳性细胞少,B组稍增多。C组术后1、3h,HIF-1α阳性产物开始增多,主要在脊髓前角神经元和少量胶质细胞中表达;术后1d达峰值,1～3d维持较高水平,此后逐渐减少;14d时仅可见少量白质中的胶质细胞。A、B组间HIF-1α阳性细胞数比较差异无统计学意义(t=1.325,P=0.137)。C组各时间点HIF-1α阳性细胞数均高于A、B组(P<0.05),C组各时间点间比较差异均有统计学意义(P<0.05)。结论 SCI后HIF-1α表达开始升高,与继发性SCI缺血缺氧密切相关,其表达规律与损伤时间具有相关性。Objective To investigate the expression pattern of hypoxia-inducible factor 1α（HIF-1α） in experimental secondary spinal cord injury（SSCI） in rats and its potential effects on SSCI.Methods A total of 66 SD rats（female or male） with weight（250± 20） g were randomly divided into 3 groups：normal control group（group A,n=6）,pseudo injury group（group B,n=6）,and spinal cord injury（SCI） group（group C,n=54）.In group A,no treatment was given as normal control.In group B,only laminectomy was applied.In group C,laminectomy was applied and static compression model of SCI was built at T10 level.The expression of HIF-1α was measured with HE and immunohistochemical staining in groups A,B（1 hour after pseudo injury）,and C（1,3,6,12 hours and 1,2,3,7,14 days after SCI）.Results All rats survived to the end of the experiment.HE staining showed that the spinal tissue of groups A and B were dense and the nucleus were round and big with light staining and clear nucleolus.The injured neuron at 1-12 hours after SCI of group C presented pyknosis and deep eosin staining.The swelling axon with bubbles and the disintegrated and disorganized medullary sheath in white matter appeared at 1-3 days after SCI.The hyperplasia of glial cells were obvious and gray matter cells were broken and apoptosis with cavities in injured spinal segment was observed at 7 and 14 days after SCI.Immunohistochemical staining showed that HIF-1α was poorly expressed in group A and increased a little in group B.The positive expression in group C increased at 3 hours after SCI,which was found in spinal cord anterior horn neurons and a small amount of ganglion cells.It reached peak at 1 day,maintained at a high level during 1-3 days and then declined.At 14 days,it appeared only in a small amount of ganglion cells of white matter.There was no significant difference in the number of HIF-1α positive cells between groups A and B（t=1.325,P=0.137）.The number of HIF-1α positive cells at each time point in group C was more than t

关 键 词：低氧诱导因子1Α 继发性脊髓损伤 缺血缺氧 大鼠 

分 类 号：R651.2[医药卫生—外科学]