C3a、C5a及其受体拮抗剂对肾小管上皮细胞β-catenin表达的影响  

The Effect of C3a,C5a and Their Receptor Antagonists on the Expression of β-catenin in Renal Tubular Cells

作　　者：刘芳[1] 苟蓉[1] 黄俊[1] 付平[1] 

机构地区：[1]四川大学华西医院肾脏内科,成都610041

出　　处：《四川大学学报（医学版）》2011年第1期74-77,共4页Journal of Sichuan University(Medical Sciences)

基　　金：四川省科技厅-攻关项目(2008SZ0182)资助

摘　　要：目的探讨补体分子C3a、C5a及其受体阻断剂(C3aRA、C5aRA)对体外培养的肾小管上皮细胞株HK-2细胞β-catenin表达的影响。方法将体外培养的HK-2细胞分成C3a组和C5a组,各组再分成4亚组:C3a组〔对照组,1μmol/L转化生长因子β1(TGF-β1)组,50nmol/LC3a组,1μmol/LC3aRA组〕;C5a组(对照组,1μmol/LTGF-β1组,50nmol/LC5a组,2.5μmol/LC5aRA组)。运用实时荧光定量PCR(RT-PCR)、Westernblot方法检测各组β-catenin的表达。结果 RT-PCR和Western blot结果显示,1μmol/LTGF-β1可以促使HK-2细胞β-catenin mRNA和蛋白质的高表达,C3a和C5a也可以刺激β-catenin mRNA和蛋白质的表达,但诱导作用较TGF-β1弱;而C3aRA、C5aRA则可以减弱C3a和C5a的刺激作用。结论 C3a、C5a可以诱导肾小管上皮细胞β-catenin mRNA和蛋白质的表达,而C3aRA、C5aRA则可以减弱C3a和C5a的刺激作用。C3a、C5a可能参与了肾小管上皮细胞肌成纤维细胞转分化得过程。Objective To investigate the effect of C3a and C5a and their receptor antagonists（C3aRA and C5aRA） on the expression of β-catenin in renal tubular epithelial cell line HK-2.Methods Cells were divided in into C3a group and C5a group which was further divided into four subgroups：C3a group（control,1 μmol/L TGF-β1,50 nmol/L C3a,1 μmol/L C3aRA）;C5a group（control,1 μmol/L TGF-β1,50 nmol/L C5a,2.5 μmol/L C5aRA）.Real time PCR and Western blot were used to detect mRNA and protein expression of β-catenin.Results Real-time PCR and Western blot demonstrated that 1 μmol/L TGF-β1 could increase the expression ofβ-catenin;C3a and C5a presented the similar inducible effect as TGF-β1,which could be blocked by C3aR antagonist and C5aR antagonist（C3aRA and C5aRA）.Conclusion Anaphylatoxin C3a and C5a can induce mRNA and protein expression of β-catenin in renal tubular epithelial cells,which could be blocked by C3aRA and C5aRA.C3a and C5a may participate in tubular epithelial-mesenchymal transition in vitro.

关 键 词：C3A C5A 肾小管上皮细胞 转化生长因子β1 Β-CATENIN 

分 类 号：R692.5[医药卫生—泌尿科学]