白细胞介素-6基因多态性与高脂血症的相关性  

Association of interleukin-6 polymorphism and hyperlipemia

作　　者：何美霞[1] 张明明[1] 刘琼[2] 华海婴[1] 巴云鹏[3] 张莉蓉[4] 

机构地区：[1]郑州大学医药科学研究院河南省医药科学研究院药理研究室,河南郑州450052 [2]中南大学湘雅医院心血管内科 [3]郑州大学第一附属医院耳鼻喉科 [4]郑州大学基础医学院药理教研室

出　　处：《中国老年学杂志》2011年第1期23-25,共3页Chinese Journal of Gerontology

基　　金：河南省杰出青年基金项目(No.074100510020)

摘　　要：目的探讨高血脂患者白细胞介素-6（IL-6）启动子-634C/G基因多态性是否为高脂血症的遗传因素。方法筛选高脂血症患者197例,健康志愿者200例。采用酚-氯仿法提取外周血DNA,PCR-RFLP技术检测IL-6基因多态性。结果高脂血症组CC、CG和GG基因型的频率分别为51.3%（101/197）,37.6%（74/197）和11.1%（22/197）,与正常对照组〔CC：65%（130/200）;CG：28.5%（57/200）;GG：6.5%（13/200）〕相比差异具有显著性（P〈0.05）。关联分析显示G等位基因是高脂血症的一个独立危险因素（OR=1.736,95%CI=1.257~2.397）。高脂血症组中,CG及GG基因型者的总胆固醇（TC）水平明显高于CC型,GG基因型高密度脂蛋白胆固醇（HDL-C）水平明显低于CC型。结论 IL-6-634 CG及GG基因型和G等位基因携带者具有高脂血症易感性。Objective To investigate the importance of the interleukin-6（IL-6） gene polymorphism in Chinese population.Methods 200 healthy controls and the 197 hyperlipemia subjects were selected.DNA was extracted from peripheral blood with the method of phenol-chloroform.IL-6 gene polymorphism was screened by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）.Results In the case subjects,the frequencies of the CC,CG and GG genotypes were 51.3%（101/197）,37.6 %（74/197） and 11.1%（22/197）,respectively.There were significant differences compared with controls（CC：65%（130/200）;CG：28.5%（57/200）and GG：6.5%（13/200））.G allele was one of independent risk factors for hyperlipemia（OR=1.736;95%CI=1.257~2.397）.GC and GG carriers showed significantly higher serum total cholesterol levels than CC carriers in hyperlipemia group.In contrast,GG carriers showed significantly lower serum high-density lipoprotein cholesterol levels than CC carriers.Conclusions CG and GG genotypes and G allele gene increase the risk of hyperlipemia.

关 键 词：IL-6-634C/G 基因多态性 高脂血症 

分 类 号：R589.2[医药卫生—内分泌]