即刻电针不同穴位对痛经模型大鼠子宫IP_3的影响  

Effects of instant electroacupuncture at the different acupoints on IP3 in the uterus tissue of dysme-norrhea model rats

作　　者：任晓暄[1] 丁喜艳[1,2] 郭孟玮[1] 赵雅芳[1] 嵇波[1] 李春华[1] 朱江[1] 张露芬[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]北京市丰台区新村社区卫生服务中心

出　　处：《中国针灸》2011年第1期60-64,共5页Chinese Acupuncture & Moxibustion

基　　金：国家重点基础研究发展计划("973"计划)资助项目:2006 CB504503

摘　　要：目的:通过比较即刻电针不同穴位对痛经模型大鼠子宫三磷酸肌醇(IP3)的影响,观察经穴效应是否存在特异性。方法:50只雌性SD大鼠随机分为盐水组、模型组、三阴交组、血海组、合谷组,每组10只。除盐水组外,其余各组大鼠均连续10d给予皮下注射苯甲酸雌二醇注射液,末次给药1h后,腹腔注射缩宫素2U/只,制备痛经大鼠模型;盐水组每日给予同等剂量的生理盐水。于第10天,后3组分别给予即刻电针"三阴交""血海""合谷"各20min,盐水组及模型组束缚20min。同时观察扭体反应,并采用酶联免疫法检测子宫IP3含量。结果:①每分钟扭体评分:与盐水组(0.311±0.253)比较,模型组(5.867±3.442)明显升高(P<0.01);血海组(2.311±0.957)亦显著升高(P<0.05);三阴交组(1.833±1.355)、合谷组(0.743±0.306)均无显著性差异(均P>0.05)。与模型组比较,各电针组均显著降低(均P<0.01)。各电针组之间比较差异均无统计学意义(均P>0.05)。②子宫IP3含量:与盐水组[(2.698±1.491)ng/mg]比较,模型组[(0.813±0.899)ng/mg]显著降低(P<0.01);血海组[(1.865±1.012)ng/mg]无显著性差异(P>0.05);三阴交组[(1.740±0.375)ng/mg]、合谷组[(0.692±0.212)ng/mg]均显著降低(P<0.05,P<0.01)。与模型组比较,合谷组无显著性差异(P>0.05);三阴交组、血海组均显著升高(均P<0.05)。三阴交组、血海组明显高于合谷组(P<0.05,P<0.01)。结论:各电针组即刻针刺在改善痛经症状上无明显差异,但在对IP3的调节上有明显经穴效应特异性,"三阴交""血海"对痛经模型大鼠IP3的效应更显著。Objective To compare the effects of instant electroacupuncture （EA） at the different acupoints on IP3 in the uterus tissue of dysmenorrhea model rats so as to investigate the specificity of acupoints. Methods Fifty female SD rats were randomly divided into a saline group, a model group, a Sanyinjiao （SP 6） group, a Xuehai （SP 10） group and a Hegu （LI 4） group, 10 rats in each group. The rats were given subcutaneous injection of Estradiol Benzoate injectio for 10 consecutive days except those in the saline group, and intraperitoneal injection of 2U Oxytocin at 1 h after the last administration to create the dysmenorrhea rats model, and the saline group was given the same dose of saline every day. On the 10th day the rats in each EA group were given EA 20 min, and the rats in the saline group and model group were bound 20 min, and the writhing response was observed at the same time. The uterine IP3 contents were detected with enzyme linked immunosorbent assay method. Results （1） Compared with （0. 311±0. 253） in the saline group, the writhing scores per minute of （5. 867± 3. 442） in the model group and （2. 311±0. 957） in the Xuehai （SP 10） group were both increased significantly （P〈0.01,P〈0.05）, and （1. 833± 1. 355） in the Sanyinjiao （SP 6） group and （0. 743±0. 306） in the Hegu （LI 4） group showed no significant differences （P〉0.05）. Compared with that in the model group, the writhing scores per minute decreased significantly （all P〈0.01） in all the EA groups, with no significant differences among all the EA groups （all P〉0.05）. （2） Compared with （2. 698±1. 491）ng/mg in the saline group, IP3 contents of the uterus of （0. 813±0. 899）ng/mg in the model group, （1.740±0.375）ng/mg in the Sanyinjiao （SP 6） group and （0. 692±0. 212）ng/mg in the Hegu （LI 4） group were all lower significantly （P〈0.05,P〈0.01）, and （0. 743±0. 306）ng/mg in the Xuehai （SP 10） group showed no significant differences

关 键 词：痛经 经穴效应特异性 电针 即刻 三磷酸肌醇 

分 类 号：R246.3[医药卫生—针灸推拿学]