安神解虑方对广泛性焦虑大鼠脑磁共振波谱变化的干预作用  

Effect of Anshen Jielu Recipe in Intervening Cerebral Metabolism in Rats with Generalized Anxiety Disorder Using Magnetic Resonance Spectroscopy

作　　者：唐启盛[1] 李宁[2] 罗斌[1] 侯秀娟[3] 黄育玲[1] 臧凤超[4] 方芳[4] 

机构地区：[1]北京中医药大学第三附属医院脑病科,北京100029 [2]北京中医药大学第三临床医学院,北京100029 [3]北京中医药大学附属东方医院风湿科,北京100078 [4]东南大学江苏省分子影像与功能影像重点实验室,南京211189

出　　处：《中国中西医结合杂志》2011年第1期62-66,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然基金资助项目(No.30772804)

摘　　要：目的研究广泛性焦虑大鼠脑代谢物的变化及安神解虑方的干预作用。方法 32只Wistar大鼠,选取8只作为正常组,其余24只采用不确定性空瓶应激的方法建立广泛性焦虑大鼠模型,随机分为模型组(蒸溜水灌胃),对照组[盐酸丁螺环酮灌胃,2.0mg/(kg·d)],治疗组[安神解虑方灌胃,12.5g/(kg.d)]每组8只,用药7天。通过高场强(7.0T)超导动物磁共振仪检测双侧海马和前额叶皮质N-乙酰天门冬氨酸(NAA)、胆碱(Cho)、肌酸(Cr)、谷氨酸(Glu)等代谢物水平,分别计算NAA、Cho、Glu与Cr的比值,观察安神解虑方的干预作用。结果广泛性焦虑大鼠存在右侧前额叶皮质NAA、Cho浓度的降低和Glu浓度的升高,安神解虑方可升高NAA和Cho的浓度,降低异常升高的Glu浓度。结论安神解虑方具有明显抗焦虑的作用,且在药物干预的早期较盐酸丁螺环酮起效快,其作用机制与调节脑组织异常代谢有关。Objective To study the metabolic change in brain of rats with generalized anxiety disorder （GAD） and the intervention effect with Anshen Jielu Recipe （AJR） on it. Methods Eight rats selected from 32 Wistar rats as normal group,the others were established as GAD model by using uncertainty empty water bottles method. Then the GAD rats were randomly divided into the model group （saline,by gastrogavage）,the control group [buspirone hydrochloride,2.0 mg/（kg·d）,by gastrogavage],the treatment group[AJR,12.5 g/（kg·d）,by gastroavage],8 in each group,all were treated for 7 days. The concentration of cerebral metabolites,including N-acetyl aspartate （NAA）,choline （Cho）,creatine （Cr） and glutamate （Glu）,in bilateral prefrontal cortex and hippocampus were measured using high-field strong super-conductivity （7.0T） animal MRI; and the ratio of NAA/Cr,Cho/Cr and Glu/Cr were calculated. The effect of AJR intervention was evaluated by changes of MRI before and after rats being treated with AJR for 7 days. Results Rats with GAD showed lowered ratios of NAA/Cr and Cho/Cr,and elevated Glu/Cr ratio in the right prefrontal cortex than those in normal rats. After AJR intervention,the abnormal changes in the three indices were restored to certain extents. Conclusions AJR has apparent anti-anxiety effect in rats with GAD,with the effect initiation faster than that in the control group. Its mechanism is probably correlated with the regulation of abnormal metabolism in the brain.

关 键 词：安神解虑方 广泛性焦虑 大鼠 磁共振波谱 海马 前额叶 

分 类 号：R285.5[医药卫生—中药学]